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TET1 non-catalytic activity shapes the chromatin-landscape that directs de novo methylation establishment in the male germline [5hmCSeal]

ABSTRACT: In the germline, DNA methylation is globally erased in primordial germ cells (PGCs), enabling establishment of sex-specific methylomes in prospermatogonia or oocytes. The catalytic activity of TET1 is required for complete demethylation in PGCs, yet sperm from Tet1-/- mice display methylation defects not explained by incomplete erasure. Instead, these defects arise from abnormal de novo methylation during development, coinciding with erosion of H3K4me3, a chromatin modification that blocks DNMT3A/3L. Using a catalytically inactive Tet1HxD mouse line, we demonstrate a non-catalytic role of TET1 in promoting H3K4me3 deposition and protecting a subset of sperm hypomethylated regions from aberrant methylation in prospermatogonia.

ORGANISM(S): Mus musculus

PROVIDER: GSE318255 | GEO | 2026/07/22

REPOSITORIES: GEO

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TET1 non-catalytic activity shapes the chromatin-landscape that directs de novo methylation establishment in the male germline [RNAseq]

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