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P. pacificus : Dauers and Dauer-Exit at 12 hour time-point vs. Mix-stage worms

ABSTRACT: Transcriptional profiling of P. pacificus worms from (1) dauer stage, or (2) dauer-exit at 12 hours stage, compared to mix-stage worms as a common reference. The goal was to determine genes regulated during dauer development and recovery or exit from dauer stage. This data was then compared to data generated for corresponding developmental stages in the C. elegans (see NCBI GEO series GSE30977) , to study evolution of developmental pathways regulating dauer development.

ORGANISM(S): Pristionchus pacificus

PROVIDER: GSE31861 | GEO | 2011/09/03

SECONDARY ACCESSION(S): PRJNA155499

REPOSITORIES: GEO

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P. pacificus : Dauers and Dauer-Exit at 12 hour time-point vs. Mix-stage worms

Project description:Transcriptional profiling of P. pacificus worms from (1) dauer stage, or (2) dauer-exit at 12 hours stage, compared to mix-stage worms as a common reference. The goal was to determine genes regulated during dauer development and recovery or exit from dauer stage. This data was then compared to data generated for corresponding developmental stages in the C. elegans (see NCBI GEO series GSE30977) , to study evolution of developmental pathways regulating dauer development. Two-condition experiments. Experiment 1 = Dauers vs. Mix-stage worms. 4 biological replicates for each condition, including 2 dye-swaps. Experiment 2 = Dauer-Exit at 12 hour time-point s vs. Mix-stage worms. 3 biological replicates for each condition, including 1 dye-swaps. Total samples from both experiments 1 and 2 = 7.

2011-09-02 | E-GEOD-31861 | biostudies-arrayexpress

C. elegans: Dauers and Dauer-Exit at 12 hour time-point vs. Mix-stage worms

2011-07-28 | GSE30977 | GEO

Pristionchus pacificus and Caenorhabditis elegans: Dauer and Dauer exit versus mix-stage comparisons

Project description:This SuperSeries is composed of the following subset Series: GSE30977: C. elegans: Dauers and Dauer-Exit at 12 hour time-point vs. Mix-stage worms GSE31861: P. pacificus : Dauers and Dauer-Exit at 12 hour time-point vs. Mix-stage worms Refer to individual Series

2012-03-22 | E-GEOD-36644 | biostudies-arrayexpress

C. elegans: Dauers and Dauer-Exit at 12 hour time-point vs. Mix-stage worms

Project description:Transcriptional profiling of C. elegans worms from (1) dauer stage, or (2) dauer-exit at 12 hours stage, compared to mix-stage worms as a common reference. The goal was to determine genes regulated during dauer development and recovery or exit from dauer stage. This data was then compared to data generated for corresponding developmental stages in the nematode Pristionchus pacificus (separate data-sets on a custom microarray platform designed by us and manufactured by Agilent) , to study evolution of developmental pathways regulating dauer development. Two-condition experiments. Experiment 1 = Dauers vs. Mix-stage worms. 4 biological replicates for each condition, including 2 dye-swaps. Experiment 2 = Dauer-Exit at 12 hour time-point s vs. Mix-stage worms. 4 biological replicates for each condition, including 2 dye-swaps. Total samples from both experiments 1 and 2 = 8.

2011-07-27 | E-GEOD-30977 | biostudies-arrayexpress

Pristionchus pacificus

Project description:P. pacificus : Dauers and Dauer-Exit at 12 hour time-point vs. Mix-stage worms

| PRJNA155499 | ENA

Effect of germline ablation in the nematode P. pacificus

Project description:Transcriptional profiling of germline-ablated P. pacificus worms versus un-ablated wild-type controls. Food source E. coli OP50 for both conditions. The goal was to identify genes that regulate the enhanced longevity observed in germline-deficient animals. One-condition experiments. Germline ablated P. pacificus versus un-ablated wild-type P. pacificus. Developmental stage = Young adults. Food source = E. coli OP50 for both conditions. 3 biological replicates for each condition, including 2 dye-swaps.

2012-04-17 | E-GEOD-37331 | biostudies-arrayexpress

Long-term imaging reveals behavioral plasticity during C. elegans dauer exit.

Project description:During their lifetime, animals must adapt their behavior to survive in changing environments. This ability requires the nervous system to adjust through dynamic expression of neurotransmitters and receptors but also through growth, spatial reorganization and connectivity while integrating external stimuli. For instance, despite having a fixed neuronal cell lineage, the nematode Caenorhabditis elegans’ nervous system remains plastic throughout its development. Here, we focus on a specific example of nervous system plasticity, the C. elegans dauer exit decision. Under unfavorable conditions, larvae will enter the non-feeding and non-reproductive dauer stage and adapt their behavior to cope with a new environment. Upon improved conditions, this stress resistant developmental stage is actively reversed to resume reproductive development. However, how different environmental stimuli regulate the exit decision mechanism and thereby drive the larva’s behavioral change is unknown. To fill this gap, we developed a new open hardware method for long-term imaging (12h) of C.elegans larvae. We identified dauer-specific behavioral motifs and characterized the behavioral trajectory of dauer exit in different environments to identify key decision points. Combining long-term behavioral imaging with transcriptomics, we find that bacterial ingestion triggers a change in neuropeptide gene expression to establish post-dauer behavior. Taken together, we show how a developing nervous system can robustly integrate environmental changes, activate a developmental switch and adapt the organism’s behavior to a new environment.

2022-09-29 | GSE214208 | GEO

RNAseq of dauer-forming C. elegans L2d in daf-2 and daf-2;daf-42

Project description:Under adverse environmental conditions, nematodes arrest into dauer, an alternative developmental stage for diapause. Dauers endure unfavorable environment and interact with host animals to access favorable environments, thus playing a critical role in the survival of both free-living and parasitic nematodes. Here, we discovered that in Caenorhabditis elegans, daf-42 is essential for development into dauer stage, as the null mutant shows lethal phenotype during dauer entry. To examine the transcriptional changes accompanied by the absence of DAF-42 during dauer entry, we performed RNA-sequencing on daf-2 and daf-2; daf-42 worms at 52 hours after egg laying (HAE) and 60 HAE, the time at which 0% and about 40% of daf-2; daf-42 mutants form dead dauer at 25 degrees Celcius, respectively. daf-2 control worms develop into dauer stage after 60 HAE, and half of its population develop into dauer by 72 HAE, when raised at 25 degrees Celcius.

2023-04-23 | GSE230353 | GEO

mRNA-seq time course of C. elegans sampled at and after release from dauer starvation at 25 degrees

Project description:We find that the 'high-confidence oscillating' genes initiate oscillations similarly as after release from L1 dauer. We performed high-throughput RNA sequencing on worms that were synchronously released from the dauer stage by refeeding on OP50. In particular, we investigate oscillatory gene expression as described in Hendriks et al., 2014 (doi: 10.1016/j.molcel.2013.12.013) and observe the re-initiation of oscillatory gene expression from stable expression with a time delay upon dauer exit. Experimental observation of the transitions between non-oscillatory and oscillatory states during the first 5 hours after dauer exit reveals that the oscillatory gene expression is arrested in a specific oscillator phase, similar to the observation we make in animals released from L1 arrest (see Meeuse et al., 2020, doi: https://doi.org/10.1101/755421).

2020-07-27 | GSE147894 | GEO

Transcription profiling of dauer gene expression in Caenorhabditis elegans

Project description:These are the 94 microarray experiments that are published in the paper: John Wang and Stuart K. Kim. Global analysis of dauer gene expression in Caenorhabditis elegans, Development 2003 130: 1621-1634. There are 94 individual microarray experiments divided into 3 broad experiments. The first experiment is a time course of dauer exit; each time course is labeled as "Dauer MTC#". The second experiment is a time course of L1 development after starvation arrest; each time couse is labeled "L1 MTC#". The final experiment is a comparison of pure dauers (0 hours) versus 12 hours after dauer exit and are labeled "Dauer Adjust". Every time course was repeated 4 times (#N)however for the dauer 4 and 7 hour time points there are only 3 replicates. For instance, all the time points labeled as "Dauer MTC#1" are from the same starting pool of dauer worms that were aliquoted into 10 fractions and analyzed at the time indicated. Every sample is compared to a common reference RNA that is used throughout all the hybridizations. In some cases there is a "-2" after the hour designation; this means the first hybridization failed for some technical reason and thus the second hybridization (same RNA) is reported.

2005-07-13 | E-SMDB-1964 | biostudies-arrayexpress

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