Single-cell RNA sequencing reveals M2-like macrophage signatures associated with mesenchymal stem cell treatment in a murine sepsis model

ABSTRACT: Mesenchymal stem cells (MSCs) have shown therapeutic potential in preclinical sepsis models, but the mechanisms remain unclear. Using a murine cecal ligation and puncture model, we performed single-cell RNA sequencing of CD45+ immune cells 6 hours after MSC administration. MSC treatment improved survival and induced transcriptional reprogramming of macrophages toward an M2-like phenotype, accompanied by reduced inflammatory signatures. These findings provide mechanistic insight into MSC-mediated immune modulation in sepsis.

ORGANISM(S): Mus musculus

PROVIDER: GSE318688 | GEO | 2026/04/01

REPOSITORIES: GEO