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Global Transcriptome analyses in Human Trophoblast Stem Cells (human TSCs) derived from placental tissues from patients suffering from idopathic recurrent pregnancy losses (RPLS).

ABSTRACT: We established RPL-Patient specific TSC lines from placental tissues collected after patients undergoing procedures at the REI clinic at the University of Kansas Medical Center. Two groups of RPL-TSC lines were established. A control RPL-TSC group, which were established from placental tissues without any apparent defect in placental ultratructure and trophoblast layers and also maintained high expressions of protein arginine methyl transferase 1 (PRMT1) in villous cytotrophoblast cells (CTBs). The second group is the experimental RPL-TSC group, which were established from RPL-Placentae with defects in placental villi structure and trophoblast layers and showed strong repression of PRMT1 expression in villous CTBs. The established RPL-TSCs were tested for PRMT1 expression. TSCs showed similar expression patterns of PRMT1 with respect to CTBs, from which they were established. Thus, RPL-TSC lines with high expression of PRMT1 are called PRMT1_high RPL-TSCs and The experimental RPL TSCs are called PRMT1_low RPL-TSCs. Four RPL-TSC lines from each group were used for global RNA-sequencing to identify global gene expression patterns.

ORGANISM(S): Homo sapiens

PROVIDER: GSE318854 | GEO | 2026/06/15

REPOSITORIES: GEO

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			Action	DRS
		Other
	GSE318854_DESeq2Out_PRMT1high_vs_PRMT1low.csv.gz	Csv
	GSE318854_final_PRMT1high_vs_PRMT1low_count_file.txt.gz	Txt

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Project description:We established RPL-Patient specific TSC lines from placental tissues collected after patients undergoing procedures at the REI clinic at the University of Kansas Medical Center. Two groups of RPL-TSC lines were established. A control RPL-TSC group, which were established from placental tissues without any apparent defect in placental ultra-structure and trophoblast layers and also maintained high expressions of Methyl Transferase-Like3 (METTL3) in villous cytotrophoblast cells (CTBs). The second group is the experimental RPL-TSC group, which were established from RPL-Placentae with defects in placental villi structure and trophoblast layers and showed strong repression of PRMT1 expression in villous CTBs. The established RPL-TSCs were tested for PRMT1 expression. TSCs showed similar expression patterns of METTL3 with respect to CTBs, from which they were established. Thus, RPL-TSC lines with high expression of METTL3 are called METTL3_high RPL-TSCs and The experimental RPL TSCs are called METTL3_low RPL-TSCs. Six METTL3_high RPL TSC lines and three METTL3_low RPL-TSC lines were used for global RNA-sequencing to identify global gene expression patterns.

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