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Mitochondrial DNA mutation triggers early-onset intestinal epithelial cell dysfunction during acute colitis [scRNA-Seq]

ABSTRACT: Mitochondrial DNA (mtDNA) mutations cause multisystem disorders, involving gastrointestinal (GI) dysfunction. However, the mechanism underlying the selection against mutant mtDNA and the impact of pathogenic mtDNA mutations on intestinal epithelial cells (IECs) remain unclear. Here, we employed mitochondrial single-cell assay for transposase-accessible chromatin with sequencing (mtscATAC-seq) to examine the heteroplasmy of IECs in m.G5081A (mt-tRNA) and m.G12918A (mt-mRNA) mutant mouse models over a two-year lifespan. Our findings reveal age-dependent purifying selection against mutant mtDNA across nine IEC subtypes, a process originating in intestinal stem cells (ISCs) that does not strengthen during cellular differentiation. Combining the analysis of single-cell RNA sequencing, we found that young mice with high m.G5081A mutation loads exhibit IEC dysfunction, including impaired enterocyte differentiation and defective Paneth cell granule formation. The m.G5081A mutant mice exhibit increased susceptibility to DSS-induced acute colitis due to compromised intestinal barrier function and impaired wound healing caused by the mtDNA mutation. Collectively, our findings reveal the cellular dynamics of mtDNA selection in IECs and demonstrate the functional consequences of mitochondrial dysfunction on intestinal health, providing insights relevant to mitochondrial disease pathogenesis and management.

ORGANISM(S): Mus musculus

PROVIDER: GSE318902 | GEO | 2026/07/30

REPOSITORIES: GEO

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Project description:Heteroplasmic pathogenic mitochondrial DNA (mtDNA) mutations are known to cause mitochondrial diseases with varied clinical symptoms. The tissue-specific selection of heteroplasmic pathogenic mtDNA mutations and its correlation with clinical and pathological outcomes remains to be elucidated. To date, negative selection of pathogenic mutations has been observed in a limited range of cell types, including female germ cells, blood cells, particularly T cells, and intestinal epithelial cells. However, evidence of positive selection in any tissue or cell type has not been reported. In this study, we utilized the DddA-derived cytosine base editor (DdCBE) technique to generate a heteroplasmic pathogenic mutant mouse model harboring a mutation in the mito-tRNA Glu anticodon arm site. Over a two-year longitudinal investigation, we assessed the mutational selection dynamics in eight solid organs and blood from this model. Our findings reveal a significant positive selection for pathogenic mtDNA in the kidney and liver. Notably, the kidney exhibited mitochondrial kidney disease during aging, reflecting the clinical kidney defects observed in patients with mito-tRNA mutations. Intriguingly, disparate selection patterns emerged within multiple cell types of kidneys, with tubular epithelial cells and podocytes presenting positive selection, whereas immune cells exhibited purifying selection. Leveraging Sc-RNA seq and Stereo-seq, we identified Clu and Spp1 as molecular markers of the high mutant kidney tubular epithelial cells and suggest that up-regulation of these markers may activate the p-AKT pathway, thereby increasing the proliferation of high mutant tubular epithelial cells, hence leads to the positive selection on this site in mutant kidneys during aging. Our comprehensive analysis provides compelling evidence for the significance of tissue-specific selection of pathogenic mtDNA mutations. This study enhances our understanding of the mechanistic links between tissue-specific selection and mitochondrial disease pathogenesis, paving the way for the development of targeted therapeutic strategies in the future.

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Mitochondrial DNA mutation triggers early-onset intestinal epithelial cell dysfunction during acute colitis [scRNA-Seq]

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