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Lipotoxic fingerprints in clinically relevant postoperative pancreatic fistula

ABSTRACT: This study investigates lipotoxic stress responses induced by postoperative pancreatic fistula (CR-POPF) effluents in non-malignant stromal cell types relevant for anastomotic healing. Postoperative drain effluents from patients undergoing pancreatoduodenectomy were characterized by gas chromatography–mass spectrometry to identify lipolysis-derived lipid species. Based on functional cytotoxicity screening, two CR-POPF effluents with reproducible biological effects were selected for transcriptomic profiling. Primary human peritoneal mesothelial cell preparations and human foreskin fibroblasts were exposed in vitro to patient-derived effluents, a defined lipolysis-derived lipid (monopalmitin), or medium control. RNA sequencing was performed to characterize stimulus-specific transcriptional stress responses. The dataset enables comparative analysis of metabolic, inflammatory, and stress-associated gene expression programs induced by distinct lipolytic stimuli in stromal and mesothelial cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE319229 | GEO | 2026/07/29

REPOSITORIES: GEO

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Lipotoxic fingerprints in clinically relevant postoperative pancreatic fistula

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