Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptional regulation by TES and TEAD1 in SNB19 glioblastoma cells: Cut&Tag

ABSTRACT: This study uses Cleavage Under Targets and Tagmentation (Cut&Tag) chromatin profiling to map genome-wide binding sites of TES (TEAD1 Epigenetic Silencer, an engineered epigenetic silencer factor consisting of KRAB-hTEAD1(1-166)-DNMT3A/3L with V5 tag) and overexpressed wild-type TEAD1 (HA-tagged) in SNB19 glioblastoma cells. Cut&Tag was performed using the CUTANA protocol (EpiCypher). Three biological replicates were prepared for TES (detected via anti-V5) and TEAD1 (detected via anti-HA), with mouse IgG and rabbit IgG as negative controls. Peak calling identified binding sites for both factors, showing that TES largely preserves the DNA-binding specificity of wild-type TEAD1 while converting it into a transcriptional repressor.

ORGANISM(S): Homo sapiens

PROVIDER: GSE319889 | GEO | 2026/02/23

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcriptional regulation by TES and TEAD1 in SNB19 glioblastoma cells: RNA-seq

Project description:This study investigates transcriptional regulation by TES (TEAD1 Epigenetic Silencer, an engineered epigenetic silencer factor targeting the YAP/TAZ-TEAD axis) in SNB19 glioblastoma cells using RNA-seq. TES was engineered by fusing the KRAB domain, the catalytic domain of DNMT3A together with DNMT3L, and the N-terminal 166 amino acids of TEAD1. RNA-seq was performed 48 hours after lentiviral transduction to identify differentially expressed genes upon TES expression compared to GFP control in three biological replicates per condition. TES expression induced widespread transcriptional repression of YAP/TAZ-dependent gene programs, with downregulated genes enriched for cell-cycle regulation and TEAD family transcription factor binding sites.

2026-02-23 | GSE319888 | GEO

Transcriptional regulation by TES and TEAD1 in SNB19 glioblastoma cells: Cut&Tag

Project description:Transcriptional regulation by TES and TEAD1 in SNB19 glioblastoma cells: Cut&Tag

| PRJNA1425481 | ENA

ZNF200 interacts with DDX17 to regulate the transcriptional activity of RBP-J. [ChIP-Seq]

Project description:In order to explore the molecular mechanism of ZNF200 in the development of NSCLC, we conducted CUT&Tag experiments in the H1299 cell line using IgG and ZNF200 antibodies. Based on previous exploratory experiments, we found an interaction between DDX17 and ZNF200. Therefore, we stably transfected DDX17-HA into H1299 cells and performed CUT&Tag experiments using IgG and ZNF200 antibodies. We then discovered that both ZNF200 and DDX17 act on the promoter region of RBP-J. Consequently, in the DDX17-HA stable transfectants of H1299,H520 and A549 cells, we knocked down ZNF200 and performed CUT&Tag experiments using HA antibodies. At the same time, we knocked down ZNF200 and DDX17 in the H520and A549 cell lines, respectively. Since H3K4me3, as an epigenetic modification, is a hallmark of the active status of gene promoter regions and is usually associated with the activation of gene expression, we subsequently used antibodies against histone H3K4me3 for the CUT&Tag experiments.

2026-02-26 | GSE309203 | GEO

Effect of PBX2 on Head and neck squamous cell carcinoma cells TU177

Project description:To investigate the role of PBX2 in Head and neck squamous cell carcinoma. We performed profiling targeting the CUT＆Tag data of the IgG and HA-PBX2 groups

2025-06-18 | GSE282361 | GEO

Transcriptional regulation by TES and TEAD1 in SNB19 glioblastoma cells: RNA-seq

Project description:Transcriptional regulation by TES and TEAD1 in SNB19 glioblastoma cells: RNA-seq

| PRJNA1425482 | ENA

Focal amplification of HOXD-harboring chromosome region is implicated in multiple-walled carbon nanotubes-induced carcinogenicity

Project description:Amplificaition of HOXD9 and HOXD13 genes was found in MWCNTs induced carcinogencity. By overexpression or silence of of HOXD9 and HOXD13 gene may alter tumorigenicity. To investigate the realted signaling pathways invovled in HOXD regulated tumorigencity, we analyzed the gene expression profile of the HOXD9 or HOXD13 overexpression HEK293 cells, which compared to vehicle expressing cells HEK293 cells was transfected with HA-tag-HOXD9 and V5-tag HOXD13 vector, and further selected by G418. Three independnent clones and mix clones of each genes were selected, and compared to corresponding tag control cell. (HA-HOXD9-mix, a, b, c v.s HA-MOCK-1, 2, 3) (V5-HOXD13-mix, a, b, c v.s V5-MOCK-1, 2, 3)

2013-10-02 | E-GEOD-46542 | biostudies-arrayexpress

Cut & Run-seq analysis for transcription factors overexpression in GI-MEN cells

Project description:Cut & Run analysis was performed in an neuroblastoma cell line to analyze DNA bindings of ASCL1-tag-HA in GI-MEN ASCL1-tag-HA cells and GI-MEN ASCL1-tag-HA+4TFs cells; analyze DNA bindings of MYCN, PHOX2B and H3K27ac in, GI-MEN 4TFs cells, and GI-MEN ASCL1-tag-HA+4TFs cells.

2023-11-21 | GSE214793 | GEO

A mucus production programme promotes classical pancreatic ductal adenocarcinoma

Project description:CUT&RUN for IgG, H3K4me3 and HA-SPDEF in human PDA HPAF-II cells and CUT&RUN HA-Spdef in murine KPC 2D FC1245 cells We performed CUT&RUN assay in human and murine PDA cells to profile the direct binding of SPDEF to target genes.

2024-01-24 | GSE250519 | GEO

Aire regulates chromatin looping by evicting CTCF from domain boundaries and favoring accumulation of cohesin on super-enhancers [CUT&Tag]

Project description:NIPBL and IgG CUT&Tag in Aire+/+ mTECs

2021-07-30 | GSE180936 | GEO

Genome-wide distribution of H3K9me3, RNA polymerase II, Rloop, DDX55 and HA-tagged DDX55 in WT and DDX55 knockout CD4 naive T cells

Project description:Genome-wide distribution of H3K9me3, RNA polymerase II, Rloop, DDX55 and HA-tagged DDX55 in WT and DDX55 knockout CD4 naive T cells were determined by cleavage under targets and tagmentation (CUT&Tag) assays followed by deep sequencing (CUT&Tag-seq) with corresponding antibodies.

2025-09-01 | GSE263249 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data