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ScRNA-seq of cerebral organoids derived from CBP mutant iPSCs

ABSTRACT: CREB-binding protein (CBP/CREBBP) is a histone acetyltransferase essential for human development. Mutations in CBP are associated with Menke–Hennekam syndrome (MKHK), a neurodevelopmental disorder. To examine the impact of a disease-associated mutation, we performed single-cell RNA sequencing (scRNA-seq) on day 32 cerebral organoids derived from isogenic wild-type and CBP R1868 mutant human induced pluripotent stem cells (iPSCs). This scRNA-seq data captures cellular heterogeneity and gene expression programs during early neurodevelopment and reveals how the CBP R1868W mutation influences lineage specification and transcriptional states in human cerebral organoids.

ORGANISM(S): Homo sapiens

PROVIDER: GSE320222 | GEO | 2026/04/21

REPOSITORIES: GEO

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RNA-seq profiling of cerebral organoids derived from CBP mutant iPSCs

Project description:CREB-binding protein (CBP/CREBBP) is a histone acetyltransferase essential for human development. Mutations in CBP are associated with Menke–Hennekam syndrome (MKHK), a neurodevelopmental disorder. To examine the impact of a disease-associated mutation, we performed RNA sequencing on cerebral organoids derived from isogenic wild-type and CBP R1868 mutant human induced pluripotent stem cells (iPSCs) at Day6, Day12, Day18 and Day30 of differentiation. This time-course RNA-seq data captures gene expression programs during early neurodevelopment and reveals how the CBP R1868W mutation influences transcriptional states in human cerebral organoids.

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