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Blocking the Gut–Liver IgA Axis Attenuates Macrophage-Mediated Inflammation and Injury in Alcohol-Associated Liver Disease [RNA-Seq]

ABSTRACT: We generated bulk 3′ mRNA-seq (QuantSeq) profiles to characterize transcriptional responses induced by immunoglobulin A (IgA) in human CD14⁺ monocytes. CD14⁺ monocytes were stimulated on plate-bound IgA (in PBS) for 24 hours or cultured on mock-coated plates incubated with PBS alone. Libraries were prepared using the QuantSeq 3′ mRNA-Seq V2 Library Prep Kit FWD (Lexogen) and sequenced as single-end 100 bp reads on an Illumina NextSeq 2000. This series includes three technical replicates per condition from a single donor.

ORGANISM(S): Homo sapiens

PROVIDER: GSE320339 | GEO | 2026/08/04

REPOSITORIES: GEO

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