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Effect of ablation of Etv4 and Etv5 from nephron progenitor cells (NPCs) and derivatives

ABSTRACT: During kidney development, Etv4 and Etv5 are essential for ureteric bud branching. They are also expressed in NPCs and developing nephrons. Here we ablated Etv4 and Etv5 from NPCs and compared the gene expression profile of mutant and control mice at embryonic day 14.5.

ORGANISM(S): Mus musculus

PROVIDER: GSE320548 | GEO | 2026/06/09

REPOSITORIES: GEO

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Transcription profiling of mouse knockouts for Etv4 and Etv5 - plusGDNF and minusGDNF comparison using U74Av2 and 430A arrays

Project description:GDNF signaling through the Ret receptor tyrosine kinase is critical for ureteric bud branching morphogenesis during kidney development yet few of the downstream genes are currently known. We find that the ETS transcription factors Etv4 and Etv5 are positively regulated by Ret signaling in ureteric bud tips. Etv4-/-Etv5+/- mice display either renal agenesis or severe hypodysplasia, while kidney development fails completely in double homozygotes. We identify several genes whose expression in the ureteric bud depends on Etv4 and Etv5, including Cxcr4, Myb, Met, Mmp14. Thus, Etv4 and Etv5 are key components of a gene network downstream of Ret that promotes and controls renal branching morphogenesis. Experiment Overall Design: 3 plus GDNF and 2 minus GDNF chips were analyzed with different pooled samples for each chip (U74Av2 and 430A). Sample comparisons and statistical analysis were performed using dChip 1.3, using the Comparison Analysis feature to identify genes differentially expressed between the two groups. The following filtering criteria were used: the “lower limit” for fold-change between the means of the +GDNF and -GDNF samples must exceed 1.2 with a 90% confidence limit, and the absolute difference between the two group means must exceed 100.

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