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Single-Cell RNA Sequencing Analysis of Proliferative and Differentiated Hepatic Organoids Generated from hPSC-Derived Hepatocyte-Like Cells, using STEMdiff™ Hepatic Organoid Growth Medium and STEMdiff™ Hepatic Organoid Differentiation Medium

ABSTRACT: Functionally relevant in vitro human hepatocyte models are critical for drug safety and efficacy screening, and liver research. However, currently available models, such as primary human hepatocytes and immortalized cell lines, often either rapidly de-differentiate or lack metabolic maturity. Human pluripotent stem cell (hPSC)-derived hepatocyte-like cells (HLCs) and hepatic organoids represent scalable and patient-representative alternatives to conventional models, particularly for long-term cell-based assays. STEMdiff™ Hepatic Organoid media are designed to support the establishment, expansion, and differentiation of proliferative and differentiated hepatic organoids, derived from HLCs generated using the STEMdiff™ Hepatocyte Kit. Here, we compare the composition of proliferative and differentiated HLC-derived hepatic organoids, cultured in STEMdiff™ Hepatic Organoid Growth Medium and STEMdiff™ Hepatic Organoid Differentiation Medium, respectively.

ORGANISM(S): Homo sapiens

PROVIDER: GSE320584 | GEO | 2026/02/26

REPOSITORIES: GEO

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Single-Cell RNA Sequencing Analysis of Proliferative and Differentiated Hepatic Organoids Generated from hPSC-Derived Hepatocyte-Like Cells, using STEMdiff™ Hepatic Organoid Growth Medium and STEMdiff™ Hepatic Organoid Differentiation Medium

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