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Expression data from BNL CL.2 liver cells when cultured with iron, RAW 264.7 macrophages or both

ABSTRACT: We found that co-culturing BNL CL.2 liver cells with RAW 264.7 macrophages increased IRP binding in the first. To further investigate this modulation we investigated the gene expression profile in BNL CL.2 cells cultured alone, with iron, with RAW 264.7 macrophages or in the presence of both iron and macrophages. This novel reconstituted liver cell-macrophage communication pathway with the present gene expression data provides a platform for addressing how macrophages participate in the iron homeostasis of liver cells and, ultimately, in systemic iron homeostasis.

ORGANISM(S): Mus musculus

PROVIDER: GSE32090 | GEO | 2016/10/22

SECONDARY ACCESSION(S): PRJNA147569

REPOSITORIES: GEO

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Expression data from BNL CL.2 liver cells when cultured with iron, RAW 264.7 macrophages or both

Project description:We found that co-culturing BNL CL.2 liver cells with RAW 264.7 macrophages increased IRP binding in the first. To further investigate this modulation we investigated the gene expression profile in BNL CL.2 cells cultured alone, with iron, with RAW 264.7 macrophages or in the presence of both iron and macrophages. This novel reconstituted liver cell-macrophage communication pathway with the present gene expression data provides a platform for addressing how macrophages participate in the iron homeostasis of liver cells and, ultimately, in systemic iron homeostasis. We used microarrays to determine the gene expression modulation in BNL CL.2 cells in response to 24h culture with 100 micromolar ferric ammonium citrate (FAC), co-culture with RAW 264.7 macrophages or both

2016-10-22 | E-GEOD-32090 | biostudies-arrayexpress

Exosomal miRNA signatures of RAW 264.7 cells

Project description:To investigate the exosomal miRNA changes under LPS treatment in RAW 264.7 cells, 2 μg/mL LPS were added into complete medium to incubate RAW 264.7 cells. And then The exosomes were isolated and tested the exosomal miRNAs change using microarray.

2023-04-30 | GSE149630 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of genome-wide binding sites of hnRNP U in BNL CL.2 cells.

Project description:Purpose:The aim of this study is to evaluate the characteristics of hnRNP U binding site. Methods: The chromatin of BNL CL.2 cells was immunoprecipitated with anti-hnRNP U(Abcam) and normal rabbit immunoglobulin G (IgG). The immunoprecipitated DNA was then subjected to various previously constructed sequencing libraries. The libraries were sequenced using the Illumina Hiseq2500. All reads were aligned to the mm9 genome browser using the bowtie with default parameters. Results: A total number of 12249 peaks that specifically binded by hnRNP U was obtained. Conclusions: Our study represents the first detailed analysis of genome-wide binding sites of hnRNP U generated by ChIP-seq technology. hnRNP U binding profiling in Mus musculus fetal liver cell line BNL CL.2 cells, and the IgG antibody was used as a negative control

2015-11-25 | E-GEOD-75335 | biostudies-arrayexpress

Comparative characterization of osteoclasts derived from murine bone marrow macrophages and RAW 264.7 cells using quantitative proteomics

Project description:Osteoclasts (OCs) are bone-resorbing cells differentiated from macrophage/monocyte precursors in response to M-CSF and RANKL. In vitro models are principally based on primary bone marrow macrophages, but RAW 264.7 cells are frequently used because they are widely available, easy to culture, and more amenable to genetic manipulation than primary cells. Increasing evidence, however, has shown that the vastly different origins of these two cell types may have important effects on cell behavior. In particular, M-CSF is prerequisite for the differentiation of BMMs, by promoting survival and proliferation and priming the cells for RANKL induction. RAW 264.7 cells readily form OCs in the presence of RANKL, but M-CSF is not required. Based on these key differences, we sought to understand their functional implications and how it might affect osteoclast differentiation and related signaling pathways. Using a robust and high-throughput proteomics strategy, we quantified the global protein changes in OCs derived from bone marrow macrophages and RAW 264.7 cells at 1, 3, and 5 days of differentiation. Correlation analysis of the proteomes demonstrated low concordance between the two cell types (R2 ≈ 0.13). Bioinformatics analysis indicate that RANKL-dependent signaling was intact in RAW 264.7 cells, but biological processes known to be dependent on M-CSF were significantly different; including cell cycle control, cytoskeletal organization, and apoptosis. RAW 264.7 cells exhibited constitutive activation of Erk and Akt that was dependent on the activity of Abelson tyrosine kinase, and the timing of Erk and Akt activation was significantly different between BMMs and RAW 264.7 cells. Our findings provide the first evidence for major differences between BMMs and RAW 264.7 cells, indicating that careful consideration is needed when using the RAW 264.7 cell line when studying M-CSF-dependent signaling and functions.

2018-11-27 | PXD009610 | Pride

Influence of hsd17b4 knockout in gene expression of RAW 264.7 cells

Project description:Hsd17b4 is a very important for beta oxidation. However, influence of hsd17b4 on gene expression in RAW 264.7 cells is unknown yet. In this study, the influence of hsd17b4 knockout on gene expression in RAW 264.7 cells was investigated.

2020-11-30 | GSE140492 | GEO

Effect of the oxidized phospholipids PGPC and POVPC on gene expression in RAW 264.7 macrophages

Project description:The effects of 1-palmitoyl-2-glutaroyl-sn-glycero-3-phosphocholine (PGPC) and 1-palmitoyl-2-(5-oxovaleroyl)-sn-glycero-3-phosphocholine (POVPC) on gene expression in RAW 264.7 macrophages were studied using >27k elements cDNA microarrays.

2014-09-16 | E-MTAB-2781 | biostudies-arrayexpress

Global gene transcriptional profiles of RAW 264.7 cells following the infection with Brucella abortus wild and mutant strains

Project description:We focused on whether transposon mutagenesis in Brucella abortus could induce difference in the trascriptional responses of RAW 264.7 cell infection model compared to the wild strain infected RAW 264.7 cells. The function of genes in Brucella abortus was analyzed through the identified differences in gene expression between RAW 264.7 cell infected with wild and mutant strains.

2016-03-16 | GSE79264 | GEO

RNA from RAW 264.7 cells treated with miR-loaded extracellular vesicles

Project description:RNA was harvested from RAW 264.7 cells treated with miR-loaded extracellular vesicles

2022-05-13 | GSE167752 | GEO

Transcription profile of intracellular H37Ra of bEnd.3 and Raw 264.7 cells

Project description:Transcription profile of intracellular H37Ra of bEnd.3 and Raw 264.7 cells 3 d.p.i

2021-07-28 | GSE162200 | GEO

Expression data from RAW 264.7 cells treated with control or Mist-targeted GapmeR

Project description:Mist is a novel lncRNA found to be downregulated in macrophages of mice with diet-induced obesity compared to control diet. The regulatory mechanisms of mouse Mist are unknown. We induced Mist knockdown in mouse macrophage cell line RAW 264.7 and used microarray to detail Mist-mediated global gene expression changes and identify distinct classes of up- and down-regulated genes.

2020-01-30 | GSE126839 | GEO

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