Ischemic vs. nephrotoxic acute renal failure, early time points (2h and 8h)

ABSTRACT: Acute renal failure (ARF) has high morbidity and mortality. In animal ARF models, effective treatments must be administered before or shortly after the insult, limiting their clinical potential. We used microarrays to identify early biomarkers that distinguish ischemic from nephrotoxic ARF, or that detect both injury types. We compared rat kidney transcriptomes 2 and 8 hours after ischemia/reperfusion and after mercuric chloride. Quality control and statistical analyses were necessary to normalize inter-experimental groups, eliminate outliers, and exclude unaltered genes. Principal component analysis revealed distinct ischemic and nephrotoxic trajectories, and clear array groupings. Therefore, we used supervised analysis, t-tests and fold changes, to compile gene lists for each group, exclusive or non-exclusive, alone or in combination. Keywords: Disease classification/time course

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE3219 | GEO | 2006/03/17

SECONDARY ACCESSION(S): PRJNA92739

REPOSITORIES: GEO