Transcriptomics

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Differential Expression and Target Gene Analysis of PBMC-Derived microRNAs as diagnostic / disease-associated biomarkers in Acute Lymphoblastic Leukemia


ABSTRACT: Clinical specimens were collected from patients with Acute Lymphoblastic Leukemia (ALL) and from non-leukemic controls. Total RNA (including small RNA) was profiled using the Affymetrix GeneChip miRNA 4.0 array to identify differentially expressed miRNAs between ALL and controls Raw CEL files were generated using standard Affymetrix GeneChip scanning and feature extraction (AGCC or equivalent). Expression values were obtained in R using RMA normalization, followed by probe filtering was applied. Differential expression was assessed with limma using an ALL vs CTRL contrast with Benjamini–Hochberg FDR correction.

ORGANISM(S): synthetic construct Homo sapiens

PROVIDER: GSE322536 | GEO | 2026/03/02

REPOSITORIES: GEO

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