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Vibrio vulnificus Wild-type vs. smcR mutant biofilm cells (V. vulnificus quorum sensing master regulator)

ABSTRACT: Two-condition experiment, Wild-type biofilm cells vs. smcR mutant biofilm cells. Biological replicates: 3 control, 3 mutant strains, independently grown and harvested. One replicate per array. A significant portion of the SmcR regulon predicted on the basis of microarray analysis results is indeed regulated by SmcR and that SmcR functions on biofilm dispersion in V. vulnificus.

ORGANISM(S): Vibrio vulnificus

PROVIDER: GSE32270 | GEO | 2011/12/31

SECONDARY ACCESSION(S): PRJNA147327

REPOSITORIES: GEO

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Vibrio vulnificus Wild-type vs. smcR mutant biofilm cells (V. vulnificus quorum sensing master regulator)

Project description:Two-condition experiment, Wild-type biofilm cells vs. smcR mutant biofilm cells. Biological replicates: 3 control, 3 mutant strains, independently grown and harvested. One replicate per array. A significant portion of the SmcR regulon predicted on the basis of microarray analysis results is indeed regulated by SmcR and that SmcR functions on biofilm dispersion in V. vulnificus. For transcriptome analysis, the V. vulnificus whole genome TwinChip, manufactured by e-biogen (Seoul, South Korea), was used.

2011-12-31 | E-GEOD-32270 | biostudies-arrayexpress

Vibrio vulnificus: Wild-type vs. smcR mutant (V. vulnificus quorum sensing master regulator)

2011-09-20 | GSE32212 | GEO

Vibrio vulnificus: Wild-type vs. smcR mutant (V. vulnificus quorum sensing master regulator)

Project description:Two-condition experiment, Wild-type vs. smcR mutant. Biological replicates: 3 control, 3 mutant strains, independently grown and harvested. One replicate per array. A significant portion of the SmcR regulon predicted on the basis of microarray analysis results is indeed regulated by SmcR and that SmcR is a global regulator in V. vulnificus. For transcriptome analysis, the V. vulnificus whole genome TwinChip, manufactured and kindly provided by the 21C Frontier Microbial Genomics and Applications Center(Daejeon, South Korea), was used.

2011-09-19 | E-GEOD-32212 | biostudies-arrayexpress

Vibrio vulnificus : biofilm cells vs. planktonic cells

Project description:The profiles of transcripts from the planktonic cells and biofilm cells of V. vulnificus were compared by using a V. vulnificus whole-genome microarray

2014-07-26 | GSE59753 | GEO

Vibrio vulnificus : biofilm cells vs. planktonic cells

Project description:The profiles of transcripts from the planktonic cells and biofilm cells of V. vulnificus were compared by using a V. vulnificus whole-genome microarray Two-condition experiment, planktonic cells vs. biofilm cells. Biological replicates: 3 control, 3 experimental, independently grown and harvested. One replicate per array. For transcriptome analysis, the V. vulnificus whole genome TwinChip, manufactured and kindly provided by the 21C Frontier Microbial Genomics and Applications Center (Daejeon, South Korea), was used.

2014-07-26 | E-GEOD-59753 | biostudies-arrayexpress

Vibrio vulnificus: Wild type vs. iscR mutant (V. vulnificus iron-sulfur cluster regulator)

2013-02-27 | GSE44679 | GEO

Vibrio vulnificus: Wild type vs. iscR mutant (V. vulnificus iron-sulfur cluster regulator)

Project description:IscR is a novel global regulator potentially contributing to the overall success in survival and pathogenesis of V. vulnificus by coordinating the regulation of various virulence factors. The profiles of transcripts from the V. vulnificus iscR mutant and the parental wild type were compared by using a V. vulnificus whole-genome microarray. Two-condition experiment: Wild type vs. iscR mutant. Biological replicates: 3 control, 3 mutant strains, independently grown and harvested. One replicate per array. For transcriptome analysis, the V. vulnificus whole genome TwinChip, manufactured and kindly provided by the 21C Frontier Microbial Genomics and Applications Center (Daejeon, South Korea), was used.

2013-02-27 | E-GEOD-44679 | biostudies-arrayexpress

European eel immune response against Vibrio vulnificus challenge

2013-03-15 | GSE45163 | GEO

Vibrio vulnificus

Project description:Vibrio vulnificus Wild-type vs. smcR mutant biofilm cells (V. vulnificus quorum sensing master regulator)

| PRJNA147327 | ENA

European eel immune response against Vibrio vulnificus challenge

Project description:Vibriosis caused by Vibrio vulnificus on eels represents an important threat for this specie under culture conditions. Development of new transcriptomic tools is essential to increase the knowledge of eel biology, that nowadays is scarcer. Therefore, using previous results obtained by 454 sequencing of the eel immune-enriched transcriptome, an eel-specific custom microarray have been designed. Gills transcriptomic pattern were analyzed as a principal portal of entry for pathogens in fish after 1h of bath infection with Vibrio vulnificus to describe gill immune response. Moreover, two different strains were used, vibro vulnificus wild type (R99) and rtx double mutant (CT285), to asses the virulence of these pathogen caused by MARTX. Adult european eels were bath infected with two Vibrio vulnificus strains, the wild type and double Rtx mutant (CT285). After 0, 3, 12h post-infection eel gills were sampled. Three individuals per experimental point were sampled, including a Control group and a Handling control group. Obtaining a total of 24 samples. The transcriptomic profile was described for each individual sample.

2013-03-15 | E-GEOD-45163 | biostudies-arrayexpress

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