Project description:Skeletal muscle cells (myoblasts - human fetal cells from 17 week old embryo) Experiment Overall Design: this experiment include 1 samples and 6 replicates
Project description:PFOS has been shown to perturb metabolism in skeletal muscle according to previous research from our lab. To determine the transciptional changes associated with PFOS exposure, C2C12 skeletal myoblasts were exposed to PFOS for 24 hours. Changes in several metabolic pathways were seen, including amino acid and glucose metabolism.
Project description:GATA4 occupancy on the mouse genome of satellite cell-derived primary myoblasts. Proliferating myoblasts cultured in growth medium were immunoprecipitated with anti-GATA4 antibody or control IgG. Precipitated genomic DNAs were subjected to next generation sequencing. Paired-end 150 bp sequence reads of GATA4-ChIP and IgG-ChIP using mouse skeletal muscle myoblasts.
Project description:transcriptional effects of mis-expression of full length (935) myocardin in growing human skeletal myoblasts Keywords: mis-expression of transcriptional co-activator
Project description:transcriptional effects of mis-expression of full length (935) myocardin in growing human skeletal myoblasts Experiment Overall Design: Compare transcriptional profiles of Ad-myocardin infected SKM5 cells with that of Ad-B-gal infected cells