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Transposon-based construction of strand-specific RNA-seq libraries

ABSTRACT: We have developed two methods for efficiently consructing RNA-seq libraries using transposition. Each method constructs high quality RNA-seq libraries when compared to standard approaches. One of the methods (Directional Tn-RNA-seq) maintains strand-of-origin information and exhibits strand specificity comparable to current approaches.

ORGANISM(S): Homo sapiens

PROVIDER: GSE32307 | GEO | 2011/10/10

SECONDARY ACCESSION(S): PRJNA147303

REPOSITORIES: GEO

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Transposon-based construction of strand-specific RNA-seq libraries

Project description:We have developed two methods for efficiently consructing RNA-seq libraries using transposition. Each method constructs high quality RNA-seq libraries when compared to standard approaches. One of the methods (Directional Tn-RNA-seq) maintains strand-of-origin information and exhibits strand specificity comparable to current approaches. RNA-seq libraries were constructed from ECC-1, a human cell line, and Universal Human Reference RNA using transposon-based and standard RNA-seq library construciton methods.

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