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Single-cell and Spatial Transcriptomic Profiling of the Bone Metastatic Microenvironment

ABSTRACT: The goal of this study is to comprehensively characterize the cellular composition, transcriptional programs, and spatial organization of the bone metastatic microenvironment and to assess how therapeutic perturbation influences tumor–microenvironment interactions. A mouse bone metastasis model was established via internal iliac artery injection of Lewis lung carcinoma cells, and metastatic lesions were monitored by in vivo imaging. Single-cell RNA sequencing and 10x Genomics Visium–based spatial transcriptomics were generated from metastatic bone tissues to provide complementary cellular and spatial resolution.

ORGANISM(S): Mus musculus

PROVIDER: GSE323357 | GEO | 2026/03/11

REPOSITORIES: GEO

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