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Analysis of gene expression in MyD88-Knockdown-Hydra vulgaris (AEP)

ABSTRACT: Analysis of MyD88 as a putative mediator in host-microbe-interactions

ORGANISM(S): Hydra vulgaris

PROVIDER: GSE32383 | GEO | 2012/10/08

SECONDARY ACCESSION(S): PRJNA147961

REPOSITORIES: GEO

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Analysis of gene expression in MyD88-Knockdown-Hydra vulgaris (AEP)

Project description:Analysis of MyD88 as a putative mediator in host-microbe-interactions MyD88-Hairpin was micro-injected in Hydra AEP embryos. The resulting Hatchling D3 showed a patchy distribution of the transgene observed by GFP. By asexual reproduction via budding and constant selection for the marker gene GFP the transgene could be driven into different cell ines. Therefore two different cultures were established. Polyps that showed no GFP-Signal anymore (control) and Polyps with both, endodermal and ectodermal transgenic cell-lines (knockdown).

2012-10-08 | E-GEOD-32383 | biostudies-arrayexpress

Hydra vulgaris

Project description:Analysis of gene expression in MyD88-Knockdown-Hydra vulgaris (AEP)

| PRJNA147961 | ENA

Intestinal microbiome adjusts the innate immune setpoint during colonization through negative regulation of MyD88

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