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Real-time quantitative PCR analysis of ARF-deficient macrophages

ABSTRACT: Macrophages are a major cellular component of all inflammatory situations, generating proinflammatory cytokines such as TNF-alpha, IL-1, and IL-6 that are central to the initiation and maintenance of inflammation. To determine whether the tumor suppressor ARF plays a role in inflammatory gene expression, we used an 84-gene RT2 PCR array to examine the expression of inflammation-associated genes in WT and ARF-deficient macrophages treated with the TLR4 ligand LPS.

ORGANISM(S): Mus musculus

PROVIDER: GSE32446 | GEO | 2011/09/29

SECONDARY ACCESSION(S): PRJNA147721

REPOSITORIES: GEO

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Real-time quantitative PCR analysis of ARF-deficient macrophages

Project description:Macrophages are a major cellular component of all inflammatory situations, generating proinflammatory cytokines such as TNF-alpha, IL-1, and IL-6 that are central to the initiation and maintenance of inflammation. To determine whether the tumor suppressor ARF plays a role in inflammatory gene expression, we used an 84-gene RT2 PCR array to examine the expression of inflammation-associated genes in WT and ARF-deficient macrophages treated with the TLR4 ligand LPS. Peritoneal macrophages from WT and ARF-deficient mice were obtained and treated with LPS (200ng/ml) for 4 hours. WT control (without stimulation n=4), WT LPS (n=4), ARF Control (n=4), ARF LPS (n=4)

2011-09-29 | E-GEOD-32446 | biostudies-arrayexpress

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Transcription profiling of mouse neural stem cells derived from Ink4a/Arf-/- vs Bmi1-/-;Ink4a/Arf-/- mutants

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Proximal interaction network of ARF GTPases

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Expression data from pulmonary Type 2 Myeloid (T2M) cells, Eosinophils, Neutrophils, and Macrophages, from IL-25 treated 4get mice

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2014-06-01 | GSE51677 | GEO

Real-time quantitative PCR analysis of human colonic HT-29 cells under insulin stimulation

Project description:Compared mRNA expression between control and insulin treated cells. HT29 cells were treated with 100nM Insulin for 24h. The RT2 Profiler PCR Arrays PAHS-30C (SA Biosciences, MD, USA) was designed to analyze 84 genes related to human insulin signaling pathway. The RT-PCR was carried out using an ABI PRISMM-BM-. 7000 Sequence Detection System (Applied Biosystems, Foster City, CA).The expression of HSD11B2 for the 3 experiments concerned was monitored in parallel by real time PCR which confirming significant downregulation by insulin. qPCR gene expression profiling. RNA from 3 separated experiments was collected.

2014-06-01 | E-GEOD-51677 | biostudies-arrayexpress

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