Transcriptional profiling of CRP-V5 and K52Q-V5 Vibrio cholerae C6706 grown in M9 minimal medium with maltose or trehalose as sole carbon source

ABSTRACT: RNA-seq was performed to compare the transcriptomes of Vibrio cholerae C6706 strains expressing V5-tagged wild-type CRP (CRP-V5) or the acetylation-mimicking mutant K52Q-V5 during growth in M9 minimal medium supplemented with 0.8% maltose or 0.8% trehalose as the sole carbon source. Overnight cultures grown in LB were washed and resuspended in the respective M9 media, then cultivated for approximately 3 hours to mid-exponential phase. Total RNA was extracted and subjected to stranded total RNA sequencing with ribosomal RNA depletion. This dataset complements ChIP-seq and RNA-seq data previously deposited under GSE310654 by examining CRP-dependent transcriptional responses under carbon source-specific conditions.

ORGANISM(S): Vibrio cholerae C6706

PROVIDER: GSE324475 | GEO | 2026/03/13

REPOSITORIES: GEO