Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression analysis of human lymphoblastoid TK6 cells exposed to the carcinogen 3-methylcholanthrene (3-MCA)

ABSTRACT: Gene expression analysis of human lymphoblastoid TK6 cells exposed to the carcinogen 3-methylcholanthrene (3-MCA) was conducted. The information entropy of the transcriptome was measured to assess whether a change with biological significance occurred in TK6 cells exposed to 3-MCA. Pathway and literature analyses for DEGs were also performed.

ORGANISM(S): Homo sapiens

PROVIDER: GSE324658 | GEO | 2026/03/17

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Homo sapiens

Project description:3-methylcholanthrene exposed TK6 cells

| PRJDB35746 | ENA

Analysis of transcriptomic responses of genotoxic and non-genotoxic stress in human lymphoblastoid TK6 cells

Project description:Purpose: Toxicogenomics analyses may provide information about DNA-damaging properties of test compounds but are not routinely used for identification of a genotoxic potential. In this study, p53 wild type TK6 cells were exposed to six food-relevant genotoxic and four non-genotoxic compounds to elucidate genotoxic mechanism. Method: Transcriptomic responses were analyzed using RNA sequencing technology

2022-03-25 | GSE185362 | GEO

DNA methylation and hydroxymethylation mediated gene expression regulation network involved in 3-methylcholanthrene induced lung cell malignant transformation

Project description:3-methylcholanthrene (3-MCA) is a typical representative PAH. It has strong toxicity and is a typical chemical carcinogen. However, the epigenetic mechanisms underlying 3-MCA-induced tumourigenesis are largely unknown. In this study, a model of the 3-MCA-induced malignant transformation of human bronchial epithelial (HBE) cells was established successfully. The profiles of gene expression and DNA methylation and hydroxymethylation were obtained and analyzed with an Illumina HiSeq 4000. A large number of differentially expressed genes were screened out, and a number of genes that may be regulated by methylation were screened through Gene ontology (GO) analysis and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analysis.

2019-12-31 | GSE142718 | GEO

TK6 toxicogenomics reference database

2015-05-01 | GSE58431 | GEO

TK6 toxicogenomics reference database

Project description:TK6 cells were exposed to various perturbations and then the transcriptome profiles were collected at 4 hours to assemble a reference database to generate a Genotoxic / Nongenotoxic classifier using the nearest shrunken centroids method. TK6 cells were exposed to various perturbations and then the transcriptome profiles were collected at 4 hours to assemble a reference database to generate a Genotoxic / Nongenotoxic classifier using the nearest shrunken centroids method.

2015-05-01 | E-GEOD-58431 | biostudies-arrayexpress

Transcription profiling by array of the effect of chemical mutagens and carcinogens on gene expression profiles in human TK6 cells

Project description:Direct and indirect- acting genotoxic carcinogens at 3 dose levels i.e., low, medium and high with and without S9 metabolic mix, were exposed to TK6 cells along with control TK6 cell cultures in- vitro. Total RNA was isolated and hybridized to illumina microarrays to assess the effects of carcinogens on genomewide gene expression in TK6 cells.

2012-06-04 | E-TABM-1223 | biostudies-arrayexpress

Dysregulation of pathways involved in the processing of cancer and microenvironment information in MCA+TPA transformed C3H/10T1/2 cells

Project description:The two-stage cell transformation assay is an in vitro model cell culture system to identify the ability of chemicals to act as initiators or promoters of cell transforma- tion and also to study the cellular and molecular mechanisms of chemically induced morphological and neoplastic cell transformation. The global gene expression profiles of 3- methylcholanthrene (MCA)+12-O-tetradecanoylphorbol-13- acetate (TPA)-transformed C3H/10T1/2 cells are not known. Therefore, we have investigated the global transcriptional profile of MCA+TPA-transformed C3H10T1/2 cells using an 8×60 k probe microarray. The study revealed a differential regulation of pathways and gene expressions. Multifold dysregulation was seen in pathways of cancer, phagosomal activity, and tumor cell microenvironment information pro- cessing systems, notably the neuroactive ligand–receptor in- teraction, actin cytoskeleton regulation, tight junction, axon guidance, and cell adhesion molecules. The genes FGF1, EIF4E1B, MAGI1,and GRIA3 showed upregulation; these encoded the pluripotent fibroblast growth factor, the transla- tion initiation factor, the tight junction scaffolding protein, and the antiapoptotic as well as the enhancer of proliferation and migration, respectively. The genes CXCL7/CXCL5/CXCL12, H2DMB1,and HSPA1A showed downregulation; these encoded the chemotactic agent protein, the protein involved

2013-04-26 | GSE41142 | GEO

Expression data from TK6 exposed to low-dose metals

Project description:We are investigating the response of human lymphoblastoid cells to low-dose exposure of environmental metals We used microarrays to detail the global programme of gene expression upon response to low-dose metals Keywords: dose TK6 cells were grown to mid log-phase and exposed to low-doses of arsenic and cadmium. RNA was collected 24 hrs after exposure.

2010-04-14 | E-GEOD-20320 | biostudies-arrayexpress

Response of human lymphoblastoid cells to HZE (iron ions) or gamma-rays

Project description:Transcriptional profiling of human lymphoblastoid TK6 cells comparing mock irradiated cells with cells exposed 24 hours previously to 1.67 Gy HZE (1 GeV/amu iron ions accelerated at the NASA Space Research Laboratory (NSRL) of Brookhaven National Laboratory) or 2.5 Gy 137Cs gamma rays. TK6 cells were mock irradiated or exposed to HZE or gamma-rays, and RNA was harvested 24 hours later. 3 biological replicates were independently grown and harvested during three different runs at the NSRL. One replicate per array.

2011-04-01 | E-GEOD-16518 | biostudies-arrayexpress

Response of human lymphoblastoid cells to HZE (iron ions) or gamma-rays

2011-04-01 | GSE16518 | GEO