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Single-cell RNA analysis of allele-specific MHC expression in peripheral blood mononuclear cells of Mauritian-origin cynomologus and Indian-origin rhesus macaques

ABSTRACT: We have employed a single-cell sequencing approach using 10X Genomics 5'scRNAseq to study expression of the Major Histocompatibility Complex (MHC) in peripheral blood mononuclear cells (PBMC) from Mauritian-origin cynomolgus and Indian-origin rhesus macaques. The MHC region has undergone a complex series of duplications in macaques relative to humans. Steady-state RNA levels of macaque MHC class I and class II transcripts vary over several orders of magnitude in whole blood and bulk PBMC. This study represents the first 5'scRNAseq analysis to quantify allele-specific levels of MHC transcripts in various PMBC subsets of macaques and provides insights into how they may shape immune responses.

ORGANISM(S): Macaca mulatta Macaca fascicularis

PROVIDER: GSE324697 | GEO | 2026/03/16

REPOSITORIES: GEO

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