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Acute High-Intensity Exercise Alters Gut Microbiota Composition and Energy Metabolism in Different Strains of Mice

ABSTRACT: The gut microbiota plays a critical role in host energy metabolism and immune regulation, yet the temporal dynamics of microbial community responses to acute physiological stressors remain poorly characterized. While exercise is recognized as a modulator of gut microbial composition, the immediate post-exercise shifts in microbial community structure across different genetic backgrounds have not been systematically examined. Here, we present a longitudinal 16S rRNA gene sequencing dataset profiling the temporal dynamics of gut microbiota following acute high-intensity exercise in two widely used laboratory mouse strains. Age-matched male BALB/c and C57BL/6J mice were subjected to a single bout of acute exercise. The colonic content samples were collected to capture early temporal responsesat. Total genomic DNA was extracted from colonic contents and the 16S rRNA V4 region was amplified and sequenced on the NovaSeq6000 platform. This dataset enables identification of strain-specific and conserved exercise-responsive microbial taxa, and supports functional predictions related to energy metabolism and intestinal homeostasis.

ORGANISM(S): mouse metagenome

PROVIDER: GSE324758 | GEO | 2026/03/31

REPOSITORIES: GEO

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			Action	DRS
		Other
	GSE324758_RAW.tar	Raw
	GSE324758_otu_table.c.relative.txt.gz	Txt
	GSE324758_otu_table.f.relative.txt.gz	Txt
	GSE324758_otu_table.g.relative.txt.gz	Txt

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Project description:The mammalian gut harbors a diverse microbial community (gut microbiota) that mainly consists of bacteria. Their combined genomes (the microbiome) provide biochemical and metabolic functions that complement host physiology. Maintaining symbiosis seems to be a key requirement for health as dysbiosis is associated with the development of common diseases. Previous studies indicated that the microbiota and the hostM-bM-^@M-^Ys epithelium signal bidirectional inducing transcriptional responses to fine-tune and maintain symbiosis. However, little is known about the hostM-bM-^@M-^Ys responses to the microbiota along the length of the gut as earlier studies of gut microbial ecology mostly used either colonic or fecal samples. This is of importance as not only function and architecture of the gut varies along its length but also microbial distribution and diversity. Few recent studies have begun to investigate microbiota-induced host responses along the length of the gut. However, these reports used whole tissue samples and therefore do not allow drawing conclusions about specificity of the observed responses. Which cells in the intestinal tissue are responsible for the microbially induced response: epithelial, mesenchymal or immune cells? Where are the responding cells located? We used using extensive microarray analysis of laser capture microdissection (LCM) harvested ileal and colonic tip and crypt fractions from germ-free and conventionally-raised mice to investigate the microbiota-induced transcriptional responses in specific and well-defined cell populations of the hostM-bM-^@M-^Ys epithelium. Ileum and colon segments were dissected from germ-free and conventionally-raised 10-12 weeks old female C57Bl/6 mice, washed and frozen as OCT blocks. Cryosections were prepared from these OCT blocks and tip/crypt fractions isolated using laser capture microdissection. To investigate the microbiota-induced transcriptional responses specific for specific subpopulations of intestinal epithelial cells, tip and crypt fractions of ileal and colonic epithelium of germ-free and conventionally-raised 10-12 weeks old female C57Bl/6 mice were harvested using laser capture microdissection and probed in an extensive microarray analysis.

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Acute High-Intensity Exercise Alters Gut Microbiota Composition and Energy Metabolism in Different Strains of Mice

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