Expression profiling of mouse hippocampus from Apex1 conditional knockout and caloric restriction models across aging

ABSTRACT: Accumulation of oxidative DNA damage is a key contributor to brain aging and cognitive decline. The DNA repair enzyme apurinic/apyrimidinic endonuclease-1 (Apex1) plays a central role in base-excision repair, but its contribution to transcriptional programs associated with brain aging remains incompletely understood. In this study, we performed genome-wide expression profiling of mouse hippocampus to examine the effects of forebrain-specific Apex1 deletion and caloric restriction on age-associated transcriptional changes. Hippocampal tissues were collected from male mice at young (8-12 weeks) and aged (48 weeks) time points across six experimental groups: young wild-type ad libitum (WT AL), young Apex1 conditional knockout ad libitum (cKO AL), aged WT AL, aged cKO AL, aged WT caloric restriction (WT CR), and aged cKO CR. Apex1 conditional knockout mice carried the genotype Camk2a-Cre+/−;Apex1^flox/flox, while hemizygous CaMKIIα-Cre+/− littermates served as age-matched controls. Microarray analyses were performed to identify transcriptional changes associated with Apex1 deficiency, aging, and dietary intervention. The dataset provides a resource for investigating molecular pathways related to genomic maintenance, metabolism, and neuronal function during brain aging.

ORGANISM(S): Mus musculus

PROVIDER: GSE324956 | GEO | 2026/03/31

REPOSITORIES: GEO