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Transcriptome profiling of Arabidopsis thaliana PsbS and β-carbonic anhydrase overexpression lines in response to bicarbonate fertilization

ABSTRACT: Photosynthetic efficiency and stress tolerance in plants are regulated by complex interactions between photoprotective mechanisms and carbon metabolism. The photosystem II subunit PsbS plays a central role in non-photochemical quenching, while β-carbonic anhydrases contribute to CO₂ conversion and pH homeostasis in chloroplasts. To investigate the potential crosstalk between these components, we performed RNA-seq analysis of Arabidopsis thaliana lines overexpressing PsbS alone or in combination with β-carbonic anhydrase genes (βCA1 and βCA2). Wild-type (Col-0), the npq4 mutant, and transgenic overexpression lines were grown under controlled conditions and treated either with water or bicarbonate fertilization. Transcriptome profiling was performed using Illumina NovaSeq sequencing of poly(A)+ RNA libraries prepared from total RNA. The dataset contains three biological replicates for each genotype and treatment combination. These data provide insight into transcriptional responses associated with altered PsbS and β-carbonic anhydrase activity and bicarbonate-induced signaling, and can be used to explore regulatory networks linking photoprotection, carbon metabolism, and stress responses in plants.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE325023 | GEO | 2026/03/21

REPOSITORIES: GEO

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