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SLC1A5 prevents aortic aneurysm and dissection by glutaminolytic-epigenetic orchestration of vascular smooth muscle cell homeostasis [CUT&Tag]

ABSTRACT: In this study, we found that H3K9ac level were reduced both in vitro (Slc1a5 knockdown VSMCs) and in vivo (Slc1a5 knockout mice). To further investigate the potential functional significance that SLC1A5-mediated H3K9ac contributed to the pathogenesis of aortic aneurysm and dissection (AAD), we performed genome-wide cleavage under targets and tagmentation (CUT&Tag) analysis to identify candidate genes regulated by H3K9ac (CST, 9649S) in Slc1a5 knockdown mouse VSMCs. Following CUT&Tag, H3K9ac associated DNAs were amplified using non-biased conditions, labeled, and sequenced with Illumina novaseq Xplus.

ORGANISM(S): Mus musculus

PROVIDER: GSE325132 | GEO | 2026/03/17

REPOSITORIES: GEO

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SLC1A5 prevents aortic aneurysm and dissection by glutaminolytic-epigenetic orchestration of vascular smooth muscle cell homeostasis [RNA-seq]

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