Expression data from active CD4 T cells
Ontology highlight
ABSTRACT:
ORGANISM(S): Mus musculus
PROVIDER: GSE32533 | GEO | 2011/10/01
SECONDARY ACCESSION(S): PRJNA147819
REPOSITORIES: GEO
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Project description:miR-17 from the miR-17-92 cluster regulate activation-induced cell death in T cells and modulate inducible regulatory T cell differentiation. We used microarrays to detail the global program of gene expression modulated by miR-17 and aim to identify the potential targets of miR-17. CD4 T cells from wild type or miR-17-92 deficient mice were activated and transduced with empty vector or that overexpressing miR-17. Potential targets of miR-17 should be upregulated in miR-17-92 deficient CD4 T cells compared to wild type CD4 T cells, and downregulated when miR-17 were reintroduced into the miR-17-92 deficient T cells.
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Comparison of mRNA sequencing results of organoids overexpressing miR-17-92 and cultures transduced with control vector, revealed a miR-17-92 expression signature. This signature appeared to be enriched in an independent series of colorectal cancers and adenomas tissues with 13q gain, confirming that miR-17-92 expression is associated with malignant progression. However, an increase in proliferation rate was not observed in miR-17-92 overexpressing adenoma organoids in vitro. In addition, subcutaneous injection of these organoids in immunodeficient mice was insufficient to cause tumor outgrowth.
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| EGAS00001005949 | EGA
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