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Reduced Accumulation of Chemokine-Specific CD4 + T Cells in the Lungs of BeO- Exposed HLA-DP2 Transgenic Mice Deficient in CCL3/4 or Treated with Novel HLA- DP2/CCL-CAR-T Cells

ABSTRACT: Chemokines play an important role in the recruitment and activation of immune cells and in forming inflammatory cellular aggregates. However, how these chemokines and chemokines- specific CD4 + T cells contribute to the pathogenesis of CBD is not well defined. RNA sequencing of CD4 + T cells showed functional heterogeneity among chemokine specific- tissue-resident CCL4 tetramer-positive CD4 + T cells (CD44 + Tet + ). CD44 + Tet + cells display increased IFNγ and IL17 gene expression compared to the CD44 - Tet - (naïve) CD4 + T cells. Over-representation analysis of genes showed pathways involved in inflammation and cholesterol metabolism are significantly increased in CD44 + Tet + cells. Next, we showed that innate immune response in Be 2+ exposed HLA-DP2 Tg WT and CCL3-deficient mice (CCL3 - /- ) was not different, while a significant decrease in total, antigen-specific CD4 + T cells and IFNγ-producing CD4 + T cells was observed in CCL3 -/- mice. We also noticed fewer peribronchovascular aggregates in the lungs of Be 2+ exposed CCL3 -/- mice, suggesting reduced inflammation. In BeO-exposed lungs, enhanced CCL3 expression was observed in macrophages and dendritic cells (DCs). Further, using bone marrow chimeric mice, we established the role of hematopoietic and DCs in controlling Be-induced CCL3 and CCL4 responses in the lungs. Besides the chemokines, we also found that HLA-DP2 Tg mice deficient in TNF-α or treated with HLA-DP-CCL4-CAR-T cells show reduced presence of chemokine-specific CD4 + T cells in the lungs. In conclusion, our findings reveal an important role of DCs and TNF-α in maintaining CCL3/4-specific CD4 + T cell responses in CBD and also provide CAR-T cells as an alternative therapy for regulating CCL3/4-specific CD4 + T cell responses in CBD.

ORGANISM(S): Mus musculus

PROVIDER: GSE325899 | GEO | 2026/05/18

REPOSITORIES: GEO

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Project description:In the marrow and lymphatic tissues, chronic lymphocytic leukemia (CLL) cells interact with accessory cells that constitute the leukemia microenvironment. In lymphatic tissues, CLL cells are interspersed with CD68+ nurselike cells (NLC) and T cells. However, the mechanism regulating co-localization of CLL cells and these accessory cells are largely unknown. To dissect the molecular cross-talk between CLL and NLC, we profiled the gene expression of CD19-purified CLL cells before and after co-culture with NLC. NLC co-culture induced high-level expression of B cell maturation antigen (BCMA) and two chemoattractants (CCL3, CCL4) by CLL cells. Supernatants from CLL-NLC co-cultures revealed high CCL3/CCL4 protein levels. B cell receptor triggering also induced a robust induction of CCL3 and CCL4 expression by CLL cells, which was almost completely abrogated by a specific Syc inhibitor, R406. High CCL3 and CCL4 plasma levels in CLL patients suggest that activation of this pathway plays a role in vivo. These studies reveal a novel mechanism of cross-talk between CLL cells and their microenvironment, namely the secretion of two T cell chemokines by CLL-NLC interaction and in response to BCR stimulation. Through these chemokines, CLL cells can recruit accessory cells, and thereby actively create a microenvironment that favors their growth and survival. Experiment Overall Design: The microarray part of this study included samples of CLL cells from 9 different patients, analyzed directly after purification and after 14 days of co-culture with Nurse like cells . Experiment Overall Design: In detail, RNA was isolated from CD19-purified CLL cells from 9 different patientsâ peripheral blood mononuclear cells (PBMC) after Ficoll separation and subsequent purification with CD19 MicroBeads and the MACSÂ® technology according to the manufacturerâs instructions (Miltenyi Biotec, Bergisch Gladbach, Germany). For comparison, the same CLL cell samples were co-cultured for 14 days with NLC (""14d NLC"").""). For co-culture with NLC, PBMC from patients with CLL were suspended in complete RPMI medium (RPMI1640 with 10% FCS, penicillin-streptomycin-glutamine, Gibco-BRL, Grand Island, NY) to a concentration of 1 x 107/ml (total 20 ml) and incubated for 14 days in 75 cm2 tissue culture flasks (Techno Plastic Products AG) as described previously3. Nonadherent lymphoid cells then were removed and the NLC layer was washed two times with phosphate-buffered saline (PBS). The complete removal of lymphocytes was verified by phase-contrast microscopy. The nonadherent cells together with the wash-fractions were then used for RNA preparation. In order to purify the CLL B cells prior to RNA isolation, CLL PBMC were passed through a 30 Âµm nylon mesh to obtain a single-cell suspension. Then CLL B cells were purified with CD19 MicroBeads. Subsequently RNA was extracted.

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Reduced Accumulation of Chemokine-Specific CD4 + T Cells in the Lungs of BeO- Exposed HLA-DP2 Transgenic Mice Deficient in CCL3/4 or Treated with Novel HLA- DP2/CCL-CAR-T Cells

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