Single-cell RNA sequencing (scRNA-seq) of murine hematopoietic stem and progenitor cells (HSPCs) and mesenchymal stromal cells (MSCs) under cardiac stress induced by transverse aortic constriction.

ABSTRACT: We performed scRNA-seq using the 10x Genomics platform to investigate how cardiac stress influences the transcriptional profiles of murine HSPCs and MSCs. HSPCs and MSCs were isolated from bone marrow of control (sham-operated) and mice subjected to transverse aortic constriction (TAC) for four weeks. Comparative analysis of single-cell transcriptomes was conducted to identify stress-induced alterations in hematopoietic differentiation and MSC states. Integrated analysis of HSPCs and MSCs enabled the characterization of cell–cell communication networks and niche remodeling associated with cardiac stress.

ORGANISM(S): Mus musculus

PROVIDER: GSE326120 | GEO | 2026/06/06

REPOSITORIES: GEO