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Effects of LPS-induced neutrophil senescence on gene expression

ABSTRACT: Neutrophils are the most abundant leukocytes in human peripheral blood but exhibit considerable heterogeneity, with distinct subsets characterized by diverse functional states. Lipopolysaccharide (LPS) has been shown to drive neutrophil senescence, a process distinct from apoptosis. However, the global transcriptional landscape of LPS-induced aged neutrophils remains largely unexplored. Here, we performed whole-transcriptome RNA sequencing (RNA-seq) on primary human neutrophils isolated from healthy donors. Neutrophils were treated with LPS to induce senescence, with untreated cells serving as controls. Our comparative transcriptomic analysis revealed that senescent neutrophils display significant upregulation of multiple genes associated with neutrophil extracellular trap (NET) formation compared to non-aged neutrophils. This dataset provides a comprehensive resource for investigating the molecular signatures underlying neutrophil senescence and its potential implications in inflammation and host defense.

ORGANISM(S): Homo sapiens

PROVIDER: GSE326268 | GEO | 2026/04/02

REPOSITORIES: GEO

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