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Identification of quantitative trait loci controlling gene expression during the innate immunity response of soybean

ABSTRACT: Microbe associated molecular pattern (MAMP)-responsive genes were identified in leaves of four different genotypes. The genotypes used in this study were: LD0-2817P; LDX01-1-65; Ripley and EF59. Leaves from three-weeks old plants were used in this study We used microarray to identified the MAMP-responsive genes in leaves of four soybean genotypes trated with 1 uM flg22 and 50 ug/ml chitin for 30 minutes

ORGANISM(S): Glycine max

PROVIDER: GSE32642 | GEO | 2011/10/06

SECONDARY ACCESSION(S): PRJNA146965

REPOSITORIES: GEO

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Identification of quantitative trait loci controlling gene expression during the innate immunity response of soybean

Project description:Microbe associated molecular pattern (MAMP)-responsive genes were identified in leaves of four different genotypes. The genotypes used in this study were: LD0-2817P; LDX01-1-65; Ripley and EF59. Leaves from three-weeks old plants were used in this study We used microarray to identified the MAMP-responsive genes in leaves of four soybean genotypes trated with 1 uM flg22 and 50 ug/ml chitin for 30 minutes Trifoliolate leaves from three-week-old plants were detached and then vacuumed infiltrated with ddiH2O for 2 min. Water-infiltrated trifoliolate leaves from five different plants of each genotype were pooled and cut into approximately 1 cm2 slices. Equal amount of leaf slices (~30 slices) were transferred into two different Petri dishes and then floated overnight on autoclaved ddiH2O. Water was removed from both Petri dishes and was replaced with 5 ml of MAMP solution [1 mM flagellin 22 (flg22) and 50 mg of crab shell chitin (called in this study as chitin)], or 5 ml of mock solution [autoclaved ddiH2O plus equivalent amount of dimethyl sulfoxide (DMSO). DMSO was included since it was contained in the solution used to dissolve the flg22 peptide]. After a 30 min treatment, mock- and MAMP-treated leaf slices were harvested into different tubes and immediately frozen in liquid nitrogen. Samples were stored at -80 until use. All procedures described above were performed under dark conditions.

2011-10-05 | E-GEOD-32642 | biostudies-arrayexpress

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Project description:To study the effect of microbe-associated molecular pattern (MAMP) treatment (flg22) on the phosphorylation of nuclear proteins, we treated Arabidopsis plants with flg22 and after isolation of nuclear proteins, we enriched for phosphopeptides and then carried out LC-MS/MS analyses. We used statistical analysis tools to identify differentially phosphorylated proteins in response to MAMP treatment in the mock and treated samples.

2022-02-28 | PXD008403 | Pride

RNA-seq of Arabidopsis thaliana seedlings exposed to the MAMP flg22 in the presence of individual commensal bacteria [MAE]

Project description:This study evaluates the transcriptome of Arabidopsis thaliana seedlings exposed to the MAMP flg22 in the presence of 35 different bacteria

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Arabidopsis CBP60g Contributes to MAMP-Induced SA Accumulation and Is Involved in Disease Resistance

Project description:Salicylic acid (SA)-induced defense responses are important factors during effector triggered immunity and microbe-associated molecular pattern (MAMP)-induced immunity in plants. This article presents evidence that a member of the Arabidopsis CBP60 gene family, CBP60g, contributes to MAMP-triggered SA accumulation. CBP60g is inducible by both pathogen and MAMP treatments. Pseudomonas syringae growth is enhanced in cbp60g mutants. Expression profiles of a cbp60g mutant after MAMP treatment are similar to those of sid2 and pad4, suggesting a defect in SA signaling. Accordingly, cbp60g mutants accumulate less SA when treated with the MAMP flg22 or a P. syringae hrcC strain that activates MAMP signaling. MAMP-induced production of reactive oxygen species and callose deposition are unaffected in cbp60g mutants. CBP60g is a calmodulin-binding protein with a calmodulin-binding domain located near the N-terminus. Calmodulin binding is dependent on Ca2+. Mutations in CBP60g that abolish calmodulin binding prevent complementation of the SA production and bacterial growth defects of cbp60g mutants, indicating that calmodulin binding is essential for the function of CBP60g in defense signaling. These studies show that CBP60g constitutes a calmodulin-dependent link between MAMP recognition and SA accumulation that is important for resistance to P. syringae. This experiment consists of three biological replicates. For each genotype, two leaves per plant were pooled from three pots to prepare total RNA.

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RNA-seq of Arabidopsis thaliana roots exposed to the MAMP flg22 in the presence of a community of commensal bacteria [RST]

Project description:This study evaluates the transcriptome of Arabidopsis thaliana roots exposed to the MAMP flg22 in the presence of a 35-member bacterial SynCom

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Transcriptome profiling of Brassica rapa subsp. pekinensis treated with immunity elicitors

Project description:Plant leaf discs were treated with plant immunity elicitors (chitin or flg22) or water for 30 or 180 minutes

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Project description:This study evaluates the ability of MpChi, an effector protein from the fungus Moniliophthora perniciosa, in suppressing the transcriptional reprogramming associated with MTI (MAMP-triggered immunity) in Nicotiana tabacum cells exposed to the chitin oligomer NAG6.

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Flg22 induces CERK1 phosphorylation

Project description:Plant cells employ cell-surface receptors to detect pathogens and launch immunity.Here we show that flg22 can induce a receptor-like kinase CERK1 phosphorylation at juxtamembrane region (JM). However, Genome-wide transcriptional reprogramming and other flg22 responses implied that CERK1 does not directly mediate flg22 signaling. Surprisingly, CERK1 JM phosphorylation can promote chitin responses and fungal resistance for plants and genome-wide transcriptional reprogramming also suggested no sign of constitutive immunity caused by this.

2019-04-08 | GSE127879 | GEO

Gene regulation in osmpk6 mutant cells expressing OsMKK4DD

Project description:OsMKK4 is a rice MAPKK and immediately activated by treatment with chitin elicitor, a fungal MAMP. OsMKK4 phosphrylate and activate OsMPK6. We compared gene expression in osmpk6 and osmpk6 complemented cells expressing active OsMKK4 using rice 44K oligoarray.

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