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Expression of placental genes in STOX1 overexpressing mice at 16.5 days of gestation versus WT mice

ABSTRACT: Two pools of three placentas from transgenic mice of the TG13 strain and WT mice were hybridized on Nimblegen Arrays. TG13 mice overexpress the gene STOX1 and have the symptoms of severe preeclamsia.

ORGANISM(S): Mus musculus

PROVIDER: GSE32660 | GEO | 2011/10/08

SECONDARY ACCESSION(S): PRJNA147041

REPOSITORIES: GEO

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Expression of placental genes in STOX1 overexpressing mice at 16.5 days of gestation versus WT mice

Project description:Two pools of three placentas from transgenic mice of the TG13 strain and WT mice were hybridized on Nimblegen Arrays. TG13 mice overexpress the gene STOX1 and have the symptoms of severe preeclamsia. Total RNA was used

2011-10-07 | E-GEOD-32660 | biostudies-arrayexpress

Maternal iron deficiency modulates placental transcriptome and proteome in mid-gestation of mouse pregnancy

Project description:Background: Maternal iron deficiency (ID) is associated with poor pregnancy and fetal outcomes. The effect is thought to be mediated by the placenta but there is no comprehensive assessment of placental response to maternal ID. Additionally, whether the influence of maternal ID on the placenta differs by fetal sex is unknown. Objectives: Our primary aim was to identify gene and protein signatures of ID mouse placentas at mid-gestation. A secondary objective was to profile the expression of iron genes in mouse placentas across gestation. Methods: We used a real-time PCR based array to determine the mRNA expression of all known iron genes in mouse placentas at embryonic day (E) 12.5, E14.5, E16.5, and E19.5 (n=3 placentas/time point). To determine the effect of maternal ID, we performed RNA sequencing and proteomics in male and female placentas from ID and iron adequate mice at E12.5 (n=8 dams/diet). Results: In female placentas, six genes including transferrin receptor (Tfrc) and solute carrier family 11 member 2 were significantly changed by maternal ID. An additional 154 genes were altered in male ID placentas. Proteomic analysis quantified 7662 proteins in the placenta. Proteins translated from iron responsive element (IRE) containing mRNAs were altered in abundance; ferritin and ferroportin 1 decreased while TFRC increased in ID placenta. Less than 4% of the significantly altered genes in ID placentas occurred both at the transcriptional and translational levels. Conclusions: Our data demonstrate that the impact of maternal ID on placental gene expression in mice is limited in scope and magnitude at mid-gestation. We provide strong evidence for IRE-based transcriptional and translational coordination of iron gene expression in the mouse placenta. Finally, we discover sexually dimorphic effects of maternal ID on placental gene expression, with more genes and pathways altered in male compared with female mouse placentas.

2021-03-12 | PXD023454 | Pride

Placental PPARg-Regulated Genes

Project description:Robust identification of placental PPARg target genes via mutliple PPARg-dependence criteria. Integration of differential expression data from Pparg-null, Rxra-null, Med1-nul and Ncoa6-null placentas and from WT and Pparg-null Trophoblast stem cells (TSC) differentiated for 2 or 4 days in the presence or absence of the PPARg agonist Rosiglitazone (Rosi). [Placentas] Three pools of three WT placentas, each vs a litter-matched pool of three Pparg-null placentas Three pools of three WT placentas, each vs a litter-matched pool of three Rxra-null placentas Three pools of three WT placentas, each vs a litter-matched pool of three Med1-null placentas Three pools of three WT placentas, each vs a litter-matched pool of three Ncoa6-null placentas [Trophoblast stem cells (TSC)] Three independent WT TSC lines differentiated for two and four days in the presence or absence of Rosi vs two independent Pparg-null TSC lines differntiated for the same durations in the presence of Rosi

2012-07-09 | E-GEOD-39233 | biostudies-arrayexpress

Uterus and placenta gene expression at 10.5 days of pregnancies in control (B6 mice) and R3 IRCS mice

Project description:R3 IRCS have a 6 Mb fragment of mouse chromosome 1 originating from Mus spretus; they display a increase rate of embryonic mortality Eight samples corresponding to two pools of three placentas, three uterus for the two strains (B6 and R3 IRCS) were hybridized on a four-quadrant Nimblegen expression array (two tissues x two types of mice x 2 duplicates)

2012-02-01 | E-GEOD-32640 | biostudies-arrayexpress

Human IUGR placentas

Project description:IUGR (Intra-Uterine Growth Restriction) refers to a condition where the foetus does not reach its growth potential in utero. It is supposed to be often linked with placental dysfunction, especially of vascular origin. In this study, 4 pools of three placentas from human normal pregnancies and 4 pools of three placentas from IUGR human pregnancies, obtained after caesarean section near normal term , were used to prepare RNA. The cDNAs prepared from these RNA were hybridized to a Nimblegen expression array in order to detect differences in gene expression between normal and pathological placentas.

2014-01-14 | E-MTAB-1956 | biostudies-arrayexpress

Expression data from BAC transgenic mice overexpressing Glo1

Project description:We generated mice with a transgenic BAC on a B6 background. The BAC contains Glo1, and the transgenic mice were found to overexpress Glo1. We performed a microarray on whole-brain RNA of adult mice to identify differentially expressed genes resulting from Glo1 overexpression.

2012-03-27 | GSE36819 | GEO

Expression data from BAC transgenic mice overexpressing Glo1

2012-03-26 | E-GEOD-36819 | biostudies-arrayexpress

Comparative analysis of mouse and human placentae across gestation reveals species-specific regulators of placental development [mouse]

Project description:In this study, we compared the genome-wide transcriptome of mouse and human placentas across gestation to identify species-specific signatures of early development. We also compared human placental signatures to purified primary cytotrophoblasts (CTB) isolated from placentae at different gestational age.

2018-02-15 | GSE100052 | GEO

Comparative analysis of mouse and human placentas across gestation reveals species-specific regulators of placental development [human]

2018-02-15 | GSE100051 | GEO

Comparative analysis of mouse and human placentae across gestation reveals species-specific regulators of placental development [Primary CTB]

2018-02-15 | GSE107824 | GEO