Transcriptomic and chromatin accessibility profiling of liver from Midn XTR conditional knockout mice

ABSTRACT: This study investigates the role of Midnolin (Midn) in regulating hepatic transcriptional and chromatin landscapes in vivo. Midn is a ubiquitin-like domain–containing nuclear protein that mediates ubiquitin-independent proteasomal degradation of transcription factors. Although Midn has been implicated in metabolic regulation and liver disease, its physiological function in normal liver remains poorly understood. To address this, we generated a liver-specific Midn conditional depletion mouse model using an eXpressed–Trapped–Restored (XTR) gene-trap strategy. In this system, Cre recombination converts the XTR allele into a Trapped (Midn-T) state, resulting in disruption of Midn expression and activation of a GFP reporter. Liver tissues from wild-type (WT) and Midn-T mice of both sexes were collected for genomic analysis. Transcriptomic profiling was performed using RNA sequencing (RNA-seq), and chromatin accessibility was assessed using ATAC sequencing (ATAC-seq). Differential gene expression and chromatin accessibility analyses were conducted to identify Midn-dependent regulatory changes in liver tissue. These data provide insight into the role of Midn in hepatic gene regulation and chromatin organization and reveal sex-dependent effects on metabolic gene expression programs.

ORGANISM(S): Mus musculus

PROVIDER: GSE326870 | GEO | 2026/08/14

REPOSITORIES: GEO