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GelMa/TPGS@Tea Polyphenols hydrogel slows down NLRP3/CASP1-mediated chondrocyte scorch death after growth plate Injury

ABSTRACT: In this paper, a GelMA/TPGS@Tea Polyphenols (TP) bioactive hydrogel system was successfully prepared. The system utilizes the injectability and mechanical support of GelMA hydrogel, combined with the temperature-sensitive gel-forming property of TPGS and the specific inhibitory effect of tea polyphenols (TP) on NLRP3 inflammatory vesicles, with the aim of solving the problem of induced bridge formation and deformity after growth plate injury in pediatric orthopaedic clinics. In vitro and in vivo experiments showed that the hydrogel system could rapidly gel and fill the defects at physiological temperature (37°C), and the TP component significantly slowed down CASP1-mediated chondrocyte pyroptosis by inhibiting the activation of NLRP3 inflammasome, thus preventing the occurrence of bone bridges. Our study provides a bioactive, injectable, and NLRP3-targeted filler material for growth plate injury repair in pediatric orthopedics, which has great potential for preventing bone bridge formation!.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE327238 | GEO | 2026/04/14

REPOSITORIES: GEO

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GelMa/TPGS@Tea Polyphenols hydrogel slows down NLRP3/CASP1-mediated chondrocyte scorch death after growth plate Injury

Project description:GelMa/TPGS@Tea Polyphenols hydrogel slows down NLRP3/CASP1-mediated chondrocyte scorch death after growth plate Injury

| PRJNA1450026 | ENA

Single cell analysis of iPSC-derived cortical organoids in biofunctionalized gelatin hydrogels

2024-07-01 | E-MTAB-14203 | biostudies-arrayexpress

Single cell analysis of iPSC-derived cortical organoids in biofunctionalized gelatin hydrogels

Project description:Human neural organoid models have become an important tool for studying neurobiology. In this work, we compared Matrigel to an N-cadherin peptide-functionalized gelatin methacryloyl hydrogel (termed GelMA-Cad) for culturing cortical neural organoids. Specifically, we compare five materials: (1) Matrigel, (2) GelMA-Cad with high crosslinker (HC), (3) GelMA-Cad with low crosslinker (LC), (4) GelMA HC and (5) GelMA LC. We determined that both mechanical properties and peptide presentation can tune cell fate and diversity in gelatin-based matrices during differentiation. Of particular note, cortical organoids cultured in GelMA-Cad produce higher numbers of neurogenic and ciliated radial glia and upper-layer excitatory neurons—an important population for modeling neurodegenerative disease—compared to GelMA and Matrigel controls.

2024-02-28 | E-MTAB-13828 | biostudies-arrayexpress

Unraveling Performance: Proteomic Insights into Photo-Crosslinkable Biopolymer Properties

Project description:Rational design of gelatin methacryloyl (GelMA) hydrogels remains limited by insufficient control over their molecular architecture, despite their broad use in bioactive scaffolds. Especially for applications involving tissue regeneration, a delicate balance is needed between mechanical stability and the ability of cells to remodel their microenvironment. In this study, we present a novel integrative workflow combining bottom-up proteomic analysis, 3D molecular modeling, and structural characterization to elucidate how chemical and physical modifications influence GelMA network formation. A 3D model was built from proteomics data identifying site-specific methacryloyl modifications on gelatin-derived COL1α1 and COL1α2 chains. Increasing methacrylic anhydride equivalents (2.5 vs. 0.5 eq) raised the degree of substitution (GelMA 96 vs. GelMA 34) and the frequency of modification, while preserving the number of modified lysines and arginines. Circular dichroism and X-ray diffraction revealed that highly substituted GelMA showed increased β-strand formation at the expense of helices and turns. Upon photo-crosslinking, networks with different crosslink densities were created and, for the first time, covalent crosslinks were identified and localized using proteomic analysis. This structural mapping demonstrated diverse crosslinking motifs and highlighted how enzyme specificity influences detection. The resulting insights connect GelMA chemistry to its crosslinking behavior and secondary structure, enabling more precise tuning of material properties. This work contributes a foundational strategy for decoding bioactive hydrogel networks and supports improved design of GelMA scaffolds for applications such as osteogenesis.

2026-01-13 | PXD068290 | Pride

Modulation of colonic inflammation in Mdr1a-/- mice by green tea polyphenols and their effects on the colon transcriptome and proteome

2012-10-18 | GSE41644 | GEO

Green tea polyphenols alleviate liver injury induced by di(2-ethylhexyl) phthalate in mice

Project description:Di (2-ethylhexyl) phthalate (DEHP) is a common plasticizer. Studies have revealed that DEHP exposure can cause liver damage. Green tea is one of the most popular beverages in China. Green tea polyphenols (GTPs) have been proven to have therapeutic effects on organ damage induced by heavy metal exposure. However, few study report on GTP relieving DEHP-induced liver damage.

2023-10-03 | GSE232128 | GEO

Project description:stylo silage tea polyphenols

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Cellular Decoding of Simultaneous Cranial Mesenchyme and Osseous Architecture Reconstruction by Scaffolds

Project description:In this study, RNA sequencing was employed to investigate the differences in gene expression levels and patterns of cells recruited into suture-bony combined calvarial injury following the implatation of distinct types of commonly used calvarial scaffolds including gelatin methacryloyl hydrogel (GelMA), porous chitosan scaffold (CTS), and polylactic acid eletrospinning membrane (PLA).

2023-12-07 | GSE249260 | GEO

Modulation of colonic inflammation in Mdr1a-/- mice by green tea polyphenols and their effects on the colon transcriptome and proteome

Project description:This study tested the anti-inflammatory potential of a green tea extract rich in polyphenols (GrTP) in the colon of the multi-drug resistance targeted mutation (Mdr1a-/-) mouse model of IBD. A colonic histological injury score was determined for each mouse to establish the effect of GrTP on inflammation. Insights into mechanisms responsible for changes in inflammation were gained using transcriptome (microarray) and proteome (2-D gel electrophoresis and LCMS protein identification) analyses. A total of 24 male Mdr1a-/- mice purchased from Taconic (Hudson, NY, USA) at 4-5 weeks of age were used for this study. 12 mice were randomly assigned to each of two different dietary groups: control (AIN-76A powdered diet), or GrTP (AIN-76A + 0.6% green tea polyphenol). At 21 and 24 weeks of age, mice were euthanized and colon samples taken for histological and microarray analyses. The total histology score (HIS) in the colon was determined according to previously described criteria. Total RNA was isolated using TRIzol reagent. Colon RNA from four Mdr1a-/- mice on the GrTP diet (with low HIS) was compared with colon RNA from four Mdr1a-/- mice on the AIN-76A diet (with high HIS). All individual RNA samples were hybridized against a common reference RNA on separate slides. The reference RNA was prepared using equimolar RNA extracts from small intestine, colon, kidney and liver of normal healthy growing Swiss mice plus RNA extracts from Swiss mouse fetuses.

2012-10-18 | E-GEOD-41644 | biostudies-arrayexpress

3D Bioprinting Lobule-like Hepatorganoids with Induced Vascularization for Orthotopic Implantation

Project description:Orthotopic implantation tumor assays are frequently employed in tumor biology, drug screening, and personalized therapy researches. However, the limited representation of a complex tumor microenvironment in these implanted animal models hampers a comprehensive understanding of tumor progression and therapeutic response. In this research, we developed a 3D bioprinting method using gelatin methacryloyl (GelMA) hydrogel loaded with a prolonged released system of vascular endothelial growth factor (VEGF), embedding hepatocellular carcinoma cells, to fabricate lobule-like hepatorganoids that faithfully mimic the architecture of hepatic lobules. Moreover, we utilized GelMA hydrogel mixed with Matrigel, containing human umbilical vein endothelial cells (HUVECs), to induce vascularization on the organoids. Orthotopic implantation in mouse model was performed, allowing for an improved simulation of the vascularization process within hepatorganoids. Compared to control group, 3D bioprinted lobule-like hepatorganoids exhibited prolonged survival in mice, while serum analysis revealed significantly elevated levels of alpha-fetoprotein (AFP). Eventually, our findings demonstrate that this system effectively forms orthotopic implantion of liver tumors and facilitates vascularization, which may notably contribute to the understanding of tumor biology, drug screening, and personalized therapy.

2025-02-12 | GSE269768 | GEO

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