Single-cell RNA sequencing of ancestral and evolved Saccharomyces cerevisiae across 20 environmental conditions
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ABSTRACT: We performed single-cell RNA sequencing (scRNA-seq) of Saccharomyces cerevisiae cells across 20 environmental conditions using a lab w303 strain (ancestral) and an evolved strain grown in KCl under continuous directed evolution (derived). Cells were grown in each of 20 stress environments (18 for the derived strain) to log phase and harvested for scRNA-seq using the 10x Genomics Chromium platform. This dataset enables comparison of the variances in transcriptional responses across environments and between ancestral and evolved strains at single-cell resolution to reveal an adaptive hierarchy in stress response prioritization that collapses under continuous selection in a constant environment
ORGANISM(S): Saccharomyces cerevisiae
PROVIDER: GSE327265 | GEO | 2026/04/10
REPOSITORIES: GEO
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