Using iCLIP-seq to analyze the distribution of XRCC5/6 on HBV RNA

ABSTRACT: In this study, we found that XRCC5/6, as pseudourindine reader proteins, can bind Ψ on HBV RNA and regulate HBV expresion. To directly analyze the binding sites of XRCC5/6 on HBV RNA, iCLIP-seq was performed in HepG2.2.15 cells using antibodies against XRCC5 and XRCC6, PUS1. The results revealed that XRCC5/6, PUS1, specifically bind 3'UTR of HBV RNA, with significant enrichment around the Ψ sites.

ORGANISM(S): Homo sapiens

PROVIDER: GSE327677 | GEO | 2026/04/20

REPOSITORIES: GEO