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Enterotoxigenic Bacteroides fragilis induces host genotype-specific colonic epithelial and immune responses in mice, sample set #1

ABSTRACT: Colorectal cancer pathogenesis involves complex interactions between multiple risk factors including somatic mutations and microbial dysbiosis. A number of individual microbiota members have been implicated in colorectal cancer, including enterotoxigenic strains of Bacteroides fragilis (ETBF). ETBF promotes inflammation in mouse models, which has been mechanistically linked to colon tumorigenesis. We hypothesized that ETBF would promote distinct patterns of colonic damage and inflammation in mice expressing different oncogenic mutations. Mice expressing mutations in the Apc tumor suppressor gene or the BRAF or Kras oncogenes were colonized with ETBF to induce acute colitis. Seven days after colonization, tissues and stools were collected to assess for colonization, epithelial damage, and local and systemic immune responses. RNA sequencing analysis revealed varied transcriptional profiles based on mouse genotype and colon region.

ORGANISM(S): Mus musculus

PROVIDER: GSE327693 | GEO | 2026/08/12

REPOSITORIES: GEO

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Enterotoxigenic Bacteroides fragilis induces host genotype-specific colonic epithelial and immune responses in mice, sample set #2: leukocyte-enriched colon prep

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