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Effect of Rv3574, Rv0047c and Rv0602c overexpression on gene expression under ATc induction in Mycobacterium smegmatis [batch 2]

ABSTRACT: transcriptional regulation on DNA damage response is essential for persistence and mutagenesis of Mtb. Rv3574, Rv0047c and Rv0602c are conducted as control regulators with comparison to Rv2282c, Rv1990c and Rv1985c. We here report Rv3574, Rv0047c and Rv0602c inhibit cell division but show no influence on DNA damage response system compared to Rv2282c, Rv1990c and Rv1985c. Overexpression of Rv3574, Rv0047c and Rv0602c does not influence the expression of genes related to oxidative stress. Complemention of tpx does not relieve the effect of Rv3574, Rv0047c and Rv0602c. Taken together, our study provides a conceptual framework for understanding how mycobacteria orchestrate the complex DDR system through redox-based transcriptional regulation.

ORGANISM(S): Mycolicibacterium smegmatis

PROVIDER: GSE327827 | GEO | 2026/05/26

REPOSITORIES: GEO

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Effect of Rv2282c, Rv1990c and Rv1985c overexpression on gene expression under ATc induction in Mycobacterium smegmatis [batch 1]

Project description:transcriptional regulation on DNA damage response is essential for persistence and mutagenesis of Mtb. We here report Rv2282c, Rv1990c and Rv1985c as regulators for DNA damage response system. Overexpression of Rv2282c, Rv1990c and Rv1985c greatly induce DNA replication and repair in a LexA-independent pathway, and inhibit cell division. Rv2282c, Rv1990c and Rv1985c induce DNA damage response by affecting redox state in mycobacterium smegmatis.Complemention of tpx relieves the effect of Rv2282c, Rv1990c and Rv1985c. Taken together, our study provides a conceptual framework for understanding how mycobacteria orchestrate the complex DDR system through redox-based transcriptional regulation.

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Project description:DosR is the regulator of a two-component system in mycobacteria that senses oxygen concentration and the redox state of the cells.

2014-07-10 | GSE56083 | GEO

Expression data of Schizosaccharomyces pombe deletions involved in DNA damage response

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2012-09-11 | E-GEOD-40747 | biostudies-arrayexpress

Overexpression on gene expression under ATc induction in Mycobacterium smegmatis

Project description:This SuperSeries is composed of the SubSeries listed below.

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DNA damage responsive microRNAs misexpressed in human cancer regulate chemotherapy sensitivity (lung cancer cell lines)

Project description:The DNA damage response (DDR) is a cellular process that protects the genetic information against DNA damage and inhibits malignant transformation. Tumor cells are characterized by an aberrant DDR, which is exploited by genotoxic chemotherapy. However, cancer cells are able to develop drug resistance, for example by modulation of the DDR. Although the regulation of the DDR has been extensively studied, not much is known about the role of microRNAs (miRNAs). Here, we describe an approach to specifically identify DDR miRNAs in human primary epithelial cells that also play a role in tumorigenesis and response to cancer therapy in corresponding cancer cells. The miRNA expression profile was determined of 12 lung cancer cell lines.

2013-05-31 | E-GEOD-47524 | biostudies-arrayexpress

DNA damage responsive microRNAs misexpressed in human cancer regulate chemotherapy sensitivity (lung tumors)

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2013-05-31 | E-GEOD-47523 | biostudies-arrayexpress

DNA damage Signaling Pathway PCR Array to profile the expression of human genes regulating in the DNA damage response (DDR) network

Project description:Human DNA Damage Signaling Pathway RT2 Profiler Array Kit (Qiagen, Valencia, CA) was used to profile the expression of key genes involved in the DDR network as per the manufacturerâs instructions. U937 cells were incubated with PXD-101 +/- MLN4924 for 16 h, after which the RT2 ProfilerTM DNA damage Signaling Pathway PCR Array was performed to profile the expression of 84 human genes involved in the DDR network.

2016-02-05 | E-GEOD-77560 | biostudies-arrayexpress

Human Fibroblast cells immortalised cells: Control vs DNA damage

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2015-09-01 | E-GEOD-67062 | biostudies-arrayexpress

RNA-seq of OPI1 knockout yeast cells and the effect of methyl methanesulfonate (MMS) treatment relative to wild-type yeast cells with or without MMS treatment

Project description:Methyl methanesulfonate (MMS) is a DNA damage agent that induces the DNA damage response (DDR). Recent findings in the literature suggest a role for phosphatidylinositol signaling in regulating the DDR. In budding yeast, inositol biosynthesis is fine-tuned by the transcriptional repressor Opi1. We conducted RNA-seq of OPI1 knockout and wild-type yeast cells in conditions with or without treatment with 0.1% MMS for 1 hour to investigate the role of Opi1 in the response to genotoxic stress.

2023-07-05 | E-MTAB-13057 | biostudies-arrayexpress

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