Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Observe the role of AQP1 gene overexpression in the RENCA cell line

ABSTRACT: Aquaporin-1 (AQP1) has been implicated in tumor progression, including cell migration, invasion, and angiogenesis in various cancers, but its specific role in renal clear cell carcinoma remains incompletely understood. To investigate the transcriptomic changes associated with AQP1 overexpression in renal cancer, we generated stable AQP1-overexpressing RENCA cells (a murine renal cell carcinoma cell line) and corresponding negative control cells using lentiviral transduction. Two weeks post-transduction, total RNA was extracted from three biological replicates per group and subjected to paired-end high-throughput RNA sequencing (RNA-seq). This dataset provides transcriptome profiles comparing AQP1-overexpressing RENCA cells to negative control cells, aiming to identify differentially expressed genes, pathways, and potential mechanisms by which AQP1 influences renal cancer biology. The data may facilitate further studies on AQP1 as a therapeutic target or biomarker in renal clear cell carcinoma.

ORGANISM(S): Mus musculus

PROVIDER: GSE327897 | GEO | 2026/05/31

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Comparison of Renca tumor cells growing intrakidney or subcutaneously

Project description:Tumors can occur in many sites in the body, but how tissues at different anatomical sites affect tumor microenvironments and their subsequent response to therapy is not known. Here we show that different tissues can cause the same tumor to develop fundamentally different microenvironments resulting in dramatic changes in responses to immunotherapy. Whereas established subcutaneous tumors could be eradicated in the majority of mice using a combination of three agonist antibodies, tumors in visceral organs responded much less. Orthotopic kidney tumors had a microenvironment with a higher frequency of alternatively-activated macrophages and their associated molecules. Neutralizing the CCL2 chemokine or IL-13 cytokine, important in macrophage biology led to a greatly improved therapeutic effect. Importantly, a significantly reduced therapeutic response of subcutaneous tumors was observed when visceral tumors were simultaneously present in the same mice. In this study we sought to compare gene expression of mouse renal cell carcinoma cells growing in the kidney to cells growing subcutaneously. Renca is a renal cell carcinoma cell line (Murphy and Hrushesky, 1973). To produce subcutaneous tumors, mice received 2 x 10^5 Renca. Orthotopic tumors were established by first surgically isolating the organ and then injecting 2 x 10^5 Renca cells intrakidney (IK) into the outer cortex of the kidney in 20 ul of PBS. Tumors were removed from mice 12 days after implantation, when approximately 20-30 mm2. Tumors were snap frozen on liquid nitrogen prior to processing for total RNA followed by analysis on Mouse Gene 1.0 chips. Kidney Renca tumors were excised from 3 BALB/c mice and subcutaneous Renca tumors were excised from another 3 BALB/c mice. cDNA was prepared and hybridized to Mouse Gene 1.0 ST chips.

2012-09-08 | E-GEOD-40679 | biostudies-arrayexpress

Next Generation Sequencing Facilitates Quantitative Analysis of Renca and B16 Murine Cancer Line Transcriptomes

Project description:Renca, a murine renal cell carcinoma, and B16F10, a murine melanoma, were transcriptionally profiled in a study designed to accompany an in vivo CRISPR screen to identify novel immunotherapy dependencies.

2020-11-25 | GSE162105 | GEO

Comparison of Renca tumor cells growing intrakidney or subcutaneously

2012-09-08 | GSE40679 | GEO

Single-nucleus RNA seq of murine renal tumors and lung metastases generated by RENCA cells overexpressing Interleukin-34

Project description:Murine renal carcinoma RENCA cells were injected in BALB/c mice to generated either primary tumours (orthotopically) or lunge metastases (through tail vein injection, or directly forming from primary tumors). Samples were snap-frozen and processed for single nucleus isolation and RNA seq analysis. Tumor cells expressed Interleukin-34 (IL34-OE) or an empty vector as control (Ctrl). The goal is to finely dissect tumor microenvironmental changes occurring in an IL34-enriched tumor microenvironment, either in primary tumors or lung metastases.

2025-06-10 | E-MTAB-14432 | biostudies-arrayexpress

Functional analysis of ANGPTL8 in clear cell renal cell carcinoma

Project description:To investigate the function of ANGPTL8 in clear cell renal cell carcinoma, Caki-1 cells overexpressing ANGPTL8 and ANGPTL8 knockout were established.

2022-05-25 | GSE203429 | GEO

Project description:Transcriptome analysis of clear cell renal carcinoma cell line overexpressing MCPIP1

| PRJEB20908 | ENA

Human renal clear-cell carcinoma cells : control vs MUC1 overexpressing cells

Project description:Transcriptional profiling of human renal clear-cell carcinoma cells comparing control unexpressing MUC1 cells (82-F7 and 82-65 samples) with MUC1 overexpressing cells (83-2 and 83-5 samples)

2014-08-25 | GSE49594 | GEO

Homo sapiens

Project description:Human renal clear-cell carcinoma cells : control vs MUC1 overexpressing cells

| PRJNA214360 | ENA

Human renal clear-cell carcinoma cells : control vs MUC1 overexpressing cells

2014-08-25 | E-GEOD-49594 | biostudies-arrayexpress

Proteomic Data of Orthotopic Renal RENCA Cell Xenografts in Balb/c Mice After Sympathetic Nerve Ablation with 6-OHDA and of the Control Group

Project description:Proteomic Data of Orthotopic Renal RENCA Cell Xenografts in Balb/c Mice After Sympathetic Nerve Ablation with 6-OHDA and of the Control Group

2025-10-15 | PXD069465 |

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data