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Single-cell RNA sequencing of intratumoral CD45+ Immune cells in a murine cancer model under chronic unpredictable mild stress (CUMS)

ABSTRACT: We performed single-cell RNA sequencing (scRNA-seq) of intratumoral CD45+ Immune cells from mice subjected to chronic unpredictable mild stress (CUMS) or control conditions. Cells were multiplexed using hashtag oligonucleotides (HTO1-HTO4, TotalSeq-C) and sequenced using the 10x Genomics 5-prime Gene Expression + Feature Barcode + B cell receptor (BCR) V(D)J workflow (Well 3). This dataset contains GEX profiles of intratumoral B cells from 4 samples (2 conditions x 2 biological replicates; 29,456 cells total). BCR V(D)J sequencing data (IGH/IGK/IGL; 1,107 contigs from 604 B cells) from the same tumor well are also provided.

ORGANISM(S): Mus musculus

PROVIDER: GSE327985 | GEO | 2026/05/11

REPOSITORIES: GEO

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