Jasmonic acid treated betelvine plants under pathogenic stress
Ontology highlight
ABSTRACT:
ORGANISM(S): Piper betle
PROVIDER: GSE328030 | GEO | 2026/05/31
REPOSITORIES: GEO
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Project description:JA-signaling in plants is manipulated by COI1 that recruits the protein complex perceiving JA signal . To determine JA correlated with the function of tobacco COI1, NtCOI1-silenced and control before and after MeJA treatment were analyzed using microarray chip.
2024-11-28 | GSE282523 | GEO
Project description:Our previous study found that the expression of HSP17.4 is closely related to the occurrence of strawberry anthracnose. In this study, we used virus-mediated gene silencing technology and found that the HSP17.4 gene-silenced ‘Sweet Charlie’ plants were more susceptible to Colletotrichum gloeosporioides than the wild type ‘Sweet Charlie’, and the level of infection was even higher than that of the susceptible cultivar ‘Benihopp’. The results of differential quantitative proteomics showed that after infection with the pathogen, the expression of the downstream response genes NPR1, TGA and PR-1 of the salicylic acid (SA) signaling pathway was fully upregulated in the wild-type ‘Sweet Charlie’, and the expression of the core transcription factor MYC2 of the jasmonic acid (JA) pathway was significantly down-regulated. The expression of the proteins encoded by these genes did not change significantly in the HSP17.4-silenced ‘Sweet Charlie’, indicating that the expression of HSP17.4 activated the up-regulation of downstream signals of SA and inhibited the JA signal pathway. The experiments that used SA, methyl jasmonate and their inhibitors to treat plants provide additional evidence that the antagonism between SA and JA regulates the resistance of strawberry plants to C. gloeosporioides.
2021-01-09 | PXD023494 |
Project description:The two-spotted spider mite, Tetranychus urticae, is one of the most significant mite pests in agriculture that can feed on more than 1,100 plant hosts, including model plants Arabidopsis thaliana and tomato, Solanum lycopersicum. In order to refine the involvement of jasmonic acid (JA) in mite-induced responses, we analyzed transcriptional changes in tomato JA signaling mutant defenseless1 (def-1) upon JA treatment and spider mite herbivory. We used microarray to assess global gene expression in Solanum lycopersicum def-1 cv. Castlemart upon jasmonic acid treatment and Tetranychus urticae attack.
2015-01-05 | GSE61075 | GEO
Project description:Defense priming sensitises plant defenses to enable a faster and stronger response to subsequent stress. Various chemicals can trigger priming, however the response remains unexplored in oak. Following treatment with salicylic acid (SA), jasmonic acid (JA), or β-aminobutyric acid (BABA), oak (Quercus robur) seedlings were infected with oak powdery mildew (Erysiphe alphitoides, PM). Whilst JA increased susceptibility to PM, BABA and SA enhanced resistance by priming callose deposition and SA-dependent gene expression, respectively. All three treatments had no impact on growth. To characterise molecular markers of priming, untargeted transcriptome and metabolome analyses were performed using RNAseq and LC-MS/MS. Differential gene expression analysis revealed around 2900, 1600, and 900 genes uniquely primed by each treatment BABA, SA, and JA, respectively. A limited number of enriched GO terms differentiated the three treatments. Meanwhile, metabolome analysis found roughly 340, 220, and 40 accumulated masses uniquely primed by BABA, SA, and JA, respectively. Pathway enrichment analysis linked BABA priming to alkaloids biosynthesis, whereas no specific pathways were identified for SA and JA priming. Our results confirm the existence of chemical-induced priming in oak and putatively identify associated molecular markers.
2025-02-28 | GSE277279 | GEO
Project description:High light (HL)-induced chloroplast retrograde signaling originates from the photosynthetic apparatus and regulates nuclear gene expression to enhance photoprotection and coordinate cell metabolism. Here, we analyzed the transcript profiles and accumulation of reactive oxygen species (ROS), stress hormones and small molecule antioxidants to investigate the signaling mechanisms operating under HL stress, and in particular during plant recovery (R) from stress under growth conditions. Exposure to HL for 15 min induced a number of singlet oxygen (1O2) and hydrogen peroxide (H2O2)-responsive genes and accumulation of an oxidative form of glutathione (GSSG) and ascorbate (DHA), the hallmarks of oxidative stress in cells. Prolonged exposure to HL resulted in accumulation of transcripts encoding oxylipin biosynthesis enzymes, leading to accumulation of 12-oxo-phytodienoic acid (OPDA) and jasmonic acid (JA). However, the expression of several JA-responsive genes, already induced by HL, peaked during the R-phase (R-sustained) together with accumulation of JA, reduced glutathione (GSH) and ascorbate (AsA), highlighting the critical role of JA signaling in restoring chloroplast redox balance following HL stress. The involvement of JA signaling in R-sustained gene expression was further confirmed by conducting experiments with JA receptor mutants (coi1). HL exposure of only 2 min was sufficient to induce some R-sustained genes, indicating rapid response of plants to changing light conditions. We propose that ROS production at HL induces the signaling cascade for early oxylipin biosynthesis and OPDA accumulation, while increased accumulation of JA in R-phase activates the genes that fully restore the glutathione metabolism, and ultimately allow recovery from short-term HL stress.
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Project description:The two-spotted spider mite, Tetranychus urticae, is one of the most significant mite pests in agriculture that can feed on more than 1,100 plant hosts, including model plants Arabidopsis thaliana and tomato, Solanum lycopersicum. In order to refine the involvement of jasmonic acid (JA) in mite-induced responses, we analyzed transcriptional changes in tomato JA signaling mutant defenseless1 (def-1) upon JA treatment and spider mite herbivory. We used microarray to assess global gene expression in Solanum lycopersicum def-1 cv. Castlemart upon jasmonic acid treatment and Tetranychus urticae attack. 1 month old def-1 tomato plants were subjected to Tetranychus urticae attack through application of 100 adult mites on a terminal leaflet of leaf 3 for 24 h or plants were sprayed with 1 mM jasmonic acid solution.
2015-01-05 | E-GEOD-61075 | biostudies-arrayexpress
Project description:Next to their essential roles in plant growth and development, phytohormones play a central role in plant immunity against pathogens. In this study we examined the role of hormones in the antagonism of the plant-pathogenic oomycete Pythium arrhenomanes against the root-knot nematode Meloidogyne graminicola in rice roots. Hormone measurements and gene expression analyses showed that the jasmonate (JA) pathway is induced early upon P. arrhenomanes infection. Exogenous application of methyl-jasmonate (MeJA) on the plant confirmed that JA is needed for basal defence against both P. arrhenomanes and M. graminicola in rice. Whereas M. graminicola suppresses root JA levels to increase host susceptibility, Pythium inoculation boosts JA accumulation up to levels that can no longer be repressed by the nematode in double-inoculated plants. Exogenous MeJA supply phenocopied the defence-inducing capacity of P. arrhenomanes against the root-knot nematode, whereas the antagonism was weakened in JA-insensitive mutants. Transcriptome analysis confirmed upregulation of JA biosynthesis and signalling genes upon P. arrhenomanes infection, and additionally revealed induction of genes involved in biosynthesis of diterpenoid phytoalexins, consistent with strong activation of the gene encoding the JA-inducible transcriptional regulator DITERPENOID PHYTOALEXIN FACTOR. Next to that, our results provide evidence for induced expression of genes encoding ERF83, and related PR proteins, as well as auxin depletion in P. arrhenomanes infected rice roots, which potentially further contributes to the reduced nematode susceptibility seen in double-infected plants.
2019-10-21 | GSE133268 | GEO
Project description:Jasmonic acid (JA) is involved in various developmental processes and defense responses against abiotic and biotic stresses. We identified JA-responsive genes in rice leaves 6-48 h after treatment using rice 44k microarray.
2011-10-06 | GSE32634 | GEO
Project description:microRNAs can play a crucial role in stress response in plants, including biotic stress. Some miRNAs are known to respond to bacterial infection. This work has addressed the role of miRNAs in Manihot esculenta (cassava)-Xanthomonas axonopodis pv. manihotis (Xam) interaction. Illumina sequencing was used for analyzing small RNA libraries from cassava tissue infected and non-infected with Xam. Cassava variety MBRA685 (resistant to Xam-CIO151) Six-week-old plants were inoculated with 36h-old cultures of the aggressive Xanthomonas axonopodis pv. manihotis strain CIO151 in both leaves and stems.
2011-12-01 | GSE29379 | GEO
Project description:Actinobacteria are prevalent in the rhizosphere and phyllosphere of diverse plant species where they help to enhance tolerance of plants against biotic and abiotic stresses. Here, we show that the plant hormones jasmonic acid (JA) and methyljasmonate (MeJA) alter growth, development and specialized metabolism of Streptomyces. Exposure of Streptomyces coelicolor to JA or MeJA led to enhanced production of the polyketide antibiotic actinorhodin. JA also exhibited toxicity towards Streptomycetaceae, whereby streptomycetes were more tolerant to JA than members of the genus Streptacidiphilus. This defensive response was associated with amino acid conjugation of JA with glutamine (Gln)-JA as the most prevalent conjugant, while conjugates with Val, Tyr, Phe and Leu/Ile were identified after longer exposure to JA. Synthetic JA conjugates failed to activate antibiotic production and showed significantly reduced toxicity. Thus, our findings shed light on a previously unknown defense mechanism deployed by Streptomyces, with amino acid conjugation protecting against the toxic effects of plant hormones. This study adds to the growing body of evidence that plant hormones can have a significant impact on members of the plant microbiome by affecting their growth, development, and secondary metabolism.
2023-08-18 | MSV000092688 | MassIVE