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Effect of docosahexaenoic acid priming of human adipose-tissue mesenchymal stromal cells used to produce secretome for regenerative applications in spinal cord injury

ABSTRACT: Bulk mRNAsec analysis was performed on human adipose-tissue mesenchymal stromal cells (ASCs) cultured in vitro after 72 hours of exposure to the omega-3 lipid docosahexaenoic acid. It was found that this lipid treatment promoted broad and specific transcriptional changes associated with metabolic and stress response pathways.

ORGANISM(S): Homo sapiens

PROVIDER: GSE328058 | GEO | 2026/05/01

REPOSITORIES: GEO

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Effect of docosahexaenoic acid priming of human adipose-tissue mesenchymal stromal cells used to produce secretome for regenerative applications in spinal cord injury

Project description:Effect of docosahexaenoic acid priming of human adipose-tissue mesenchymal stromal cells used to produce secretome for regenerative applications in spinal cord injury

| PRJNA1453591 | ENA

Transcription profiling of two human colon cancer cell lines treated with n-3 PUFA docosahexaenoic acid for 3 different time points

Project description:Several studies indicate that omega (n)-3 fatty acids may have a potential in the prevention and treatment of cancer. In this study the colon cancer cell lines SW480 and SW620 were treated with the n-3 PUFA DHA (Docosahexaenoic acid) for 3 different time points (12, 24 and 48h). The experiments were performed in three independent replicates (1-3), RNA isolated and used for Affymetrix gene expression analysis.

2008-04-01 | E-MEXP-1014 | biostudies-arrayexpress

Ferroptosis Induced by Omega-3 Docosahexaenoic Acid (DHA) and Erastin in Control MDA-MB-231 Cells (sh-LUC) and MDA-MB-231 Cells with knockdown of IGFBP6 gene (sh-IGFBP6)

Project description:In this study, we further elucidate the mechanism of action of one of the most effective antitumor PUFAs, ω-3 docosahexaenoic acid, on MDA-MB-231 breast cancer cells, including those with IGFBP6 knockdown. Additionally, we analyzed the effect of reduced IGFBP6 gene expression on the mechanism of ferroptosis in MDA-MB-231 breast cancer cells under the influence of the canonical ferroptosis inducer erastin.

2025-04-02 | GSE282896 | GEO

Differential effects of krill oil and fish oil on the hepatic transcriptome in mice

Project description:Dietary supplementation with ω-3 polyunsaturated fatty acids (ω-3 PUFAs), specifically the fatty acids docosahexaenoic acid (DHA; 22:6 ω-3) and eicosapentaenoic acid (EPA; 20:5 ω-3), is known to have beneficial health effects including improvements in glucose and lipid homeostasis and modulation of inflammation. To evaluate the efficacy of two different sources of ω-3 PUFAs, we performed gene expression profiling in the liver of mice fed diets supplemented with either fish oil or krill oil. We found that ω-3 PUFA supplements derived from a phospholipid krill fraction (krill oil) downregulated the activity of pathways involved in hepatic glucose production as well as lipid and cholesterol synthesis. The data also suggested that krill oil-supplementation increases the activity of the mitochondrial respiratory chain. Surprisingly, an equimolar dose of EPA and DHA derived from fish oil modulated fewer pathways than a krill oil-supplemented diet and did not modulate key metabolic pathways regulated by krill oil, including glucose metabolism, lipid metabolism and the mitochondrial respiratory chain. Moreover, fish oil upregulated the cholesterol synthesis pathway, which was the opposite effect of krill supplementation. Neither diet elicited changes in plasma levels of lipids, glucose or insulin, probably because the mice used in this study were young and were fed a low fat diet. Further studies of krill oil supplementation using animal models of metabolic disorders and/or diets with a higher level of fat may be required to observe these effects. Twenty-one microarrays: three diets (CO, FO, KO) x seven mice per diet x one microarray per mouse

2011-07-21 | E-GEOD-29572 | biostudies-arrayexpress

A High Docosahexaenoic Acid Diet Alters the Lung Inflammatory Response to Acute Dust Exposure

Project description:Agricultural workers are at risk for the development of acute and chronic lung diseases due to their exposure to organic agricultural dusts. A diet intervention using the omega-3 fatty acid docosahexaenoic acid (DHA) has been shown to be an effective therapeutic approach for alleviating a dust-induced inflammatory response. We thus hypothesized a high-DHA diet would alter the dust-induced inflammatory response through the increased production of specialized pro-resolving mediators (SPMs). Mice were pre-treated with a DHA-rich diet 4 week s before being intranasally challenged with a single dose of an extract made from dust collected from a concentrated swine feeding operation (HDE ). This omega-3-fatty-acid-rich diet led to reduced arachidonic acid levels in the blood, enhanced macrophage recruitment, and increased the production of the DHA-derived SPM Resolvin D1 (RvD1) in the lung following HDE exposure. An assessment of transcript-level changes in the immune response demonstrated significant differences in immune pathway activation and alterations of numerous macrophage-associated genes among HDE-challenged mice fed a high DHA diet. Our data indicate that consuming a DHA-rich diet leads to the enhanced production of SPMs during an acute inflammatory challenge to dust, supporting a role for dietary DHA supplementation as a potential therapeutic strategy for reducing dust-induced lung inflammation.

2020-08-03 | GSE155539 | GEO

NASH treatment study (Ldlr-/- mice on Western diet treated with/without omega-3 fatty acids)

Project description:Mice on a reference (RD) or western diet (WD) supplemented with olive oil or the omega-3 fatty acid docosahexaenoic acid DHA

2022-10-13 | GSE215225 | GEO

Dental Stem Cell-Derived Secretome/Conditioned Medium: The Future for Regenerative Therapeutic Applications.

Project description: Not available

| S-EPMC7013327 | biostudies-literature

Differential effects of krill oil and fish oil on the hepatic transcriptome in mice

2011-07-22 | GSE29572 | GEO

NASH prevention study (Ldlr-/- mice on Western diet treated with/without omega-3 fatty acids)

Project description:Mice on a reference (RD) or western diet (WD) supplemented with olive oil or omega-3 fatty acids (eicosapentaenoic acid [EPA] and/or docosahexaenoic acid [DHA])

2022-10-13 | GSE215224 | GEO

The protective effect of docosahexaenoic acid on mitochondria in a SH-SY5Y model of rotenone-induced toxicity

Project description:Polyunsaturated fatty acids in particular omega-3 fatty acids, such as docosahexaenoic acid (DHA) are essential nutrients and components of the plasma membrane. They are involved in various processes, among them synaptic development, functionality, integrity and plasticity and are therefore thought to have general neuroprotective properties. Considerable research evidence further supports beneficial effects of omega-3 fatty acids specifically on mitochondrial structure and function through their antioxidant and anti-apoptotic properties, which makes it an attractive addition in treatment options for neurodegenerative disorders, in which mitochondrial alterations are commonly observed. However, so far precise information on the underlying protective mechanisms are still lacking. Thus in this study, we utilized the most common neuronal cell line (SH-SY5Y) and induced mitochondrial oxidative stress through the addition of rotenone. To study the potential protective effect of DHA, cells were additionally pre-treated with DHA prior rotenone administration. By combining SILAC labelling, mitochondria enrichment, and subsequent proteomic analyses, we aimed to determine the capacity of DHA to alleviate mitochondrial oxidative stress in vitro and further shed light on molecular mechanisms contributing to the proposed neuroprotective effect. Indeed we confirmed a general increase in intensity of mitochondrial proteins in DHA pre-treated cells. Furthermore, we were able to define a compensatory mechanism by which the inhibition of complex I is overcome by an increased activity of the fatty acid beta oxidation in response to DHA pre-treatment.

2025-02-24 | PXD049302 | Pride

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