Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Pre-rRNA chromatin binding in mouse pachytene spermatocytes [ChIRP-seq]

ABSTRACT: We report the genome-wide pre-rRNA-associated chromatin in mouse pachytene spermatocytes using ChIRP-seq following immunoprecipitation with pre-rRNA probes.

ORGANISM(S): Mus musculus

PROVIDER: GSE328328 | GEO | 2026/05/28

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

BMH21 treatment disrupts pre-rRNA in the XY body of pachytene spermatocytes and alters genome accessibility (ATAC-seq)

Project description:ATAC-seq analysis of mouse pachytene spermatocytes following BMH21 treatment, which disrupts pre-rRNA within the XY body. BMH21 treatment induces chromatin accessibility changes compared to DMSO control. Two biological replicates per condition.

2026-05-28 | GSE328202 | GEO

Gene expression of pachytene-stage spermatocytes during male meiosis in Mus musculus [RNA-Seq]

Project description:Inhibition of pre‑rRNA transcription by BMH21 (12 h) in ex vivo mouse testicular single‑cell suspensions, followed by FACS isolation of pachytene spermatocytes (Hoechst 33342) and RNA‑seq. This dataset is designed to assess whether perturbing pre‑rRNA accumulation within the XY body affects the transcriptional landscape of pachytene spermatocytes, particularly regarding meiotic sex chromosome inactivation (MSCI). Comparison of BMH21‑treated vs control cells identifies differentially expressed autosomal and sex‑linked genes, providing insights into the role of nucleolar activity in sex chromosome regulation during male meiosis.

2026-05-28 | GSE328326 | GEO

Expression profiling of isolated populations of prepachytene spermatocytes, pachytene spermatocytes and spermatids of PWD and B6 males

2014-06-01 | GSE49444 | GEO

Expression profiling of isolated populations of prepachytene spermatocytes, pachytene spermatocytes and spermatids of PWD and B6 males

Project description:Expression profiling of isolated populations of prepachytene spermatocytes (LP), pachytene spermatocytes (RP) and spermatids (ST) from PWD and B6 was performed to study the genome wide variation in gene expression between two mouse subspecies. To evaluate the transcriptional difference between B6 and PWD in during meiosis, we compared their transcriptomes in sorted populations of pre-pachytene primary spermatocytes (Leptonema, Zygotene and Pachytene), pachytene spermatocytes (Mid-late pachytene and diplotene) and spermatids. Populations of pre-pachytene spermatocytes, pachytene spermatocytes and spermatids were isolated from PWD and B6 mice. All populations were isolated in duplicates from two animals. Hence, RNA was prepared from 12 samples (= 2 strains x 3 tissues x 2 replicas) and was then subject to labeling and hybridization on microarray chips.

2014-06-01 | E-GEOD-49444 | biostudies-arrayexpress

Characterization of ZFP541's occupency sites on genome in pachytene and diplotene spermatocytes

Project description:The DSB-machinery, which induces the programmed DNA double-strand breaks (DSBs) in leptotene and zygotene stages during meiosis, needs to be kept in silence after the initiation of pachytene stage to prevent the activation of DSB checkpoint that may lead to meiotic arrest or apoptosis of germ cells. However, the mechanisms underlying this repression remain largely unknown. Here, we report that ZFP541, a germ cell-specific zinc finger protein, is responsible for the suppression of DSBs formation at late pachytene. Lack of Zfp541 in mice leads to generation of DSBs in late pachytene spermatocytes by DSB formation related-proteins and causes male infertility due to meiotic failure. Plated-based scRNA-seq of Zfp541-/- spermatocytes revealed that ZFP541 negatively regulates many meiotic prophase genes, including genes for DSB formation and their upstream transcriptional regulators, in late pachytene spermatocytes. These results were confirmed by 10x single-cell RNA-seq data on spermatogenesis of Zfp541-/- testes, which suggested that Zfp541 is required for repressing the activation of pre-pachytene gene expression programs from early to late pachytene. ZFP541 ChIP-seq on pachytene and diplotene spermatocytes demonstrated that ZFP541 occupies the promoters of meiosis initiators (e.g., Meiosin and Rxra) and a subset of their downstream genes to repress their transcription, and thus prevent the reactivation of pre-pachytene gene expression programs in pachytene spermatocytes. Thus, our results not only revealed the role of ZFP541 in maintaining the repression of pre-pachytene transcriptional programs in pachytene spermatocytes but also provide new insight into the regulation of meiotic progression by timely turning off pre-pachytene genes.

2021-04-16 | GSE172160 | GEO

miRNA expression profiling of isolated populations of prepachytene spermatocytes, pachytene spermatocytes and spermatids of PWD and B6 male mice

Project description:MiRNA expression profiling of isolated populations of prepachytene spermatocytes (LP), pachytene spermatocytes (RP) and spermatids (ST) from PWD and B6 was performed to study the genome wide variation in miRNA expression between two mouse subspecies. Populations of pre-pachytene spermatocytes, pachytene spermatocytes and spermatids were isolated from PWD and B6 mice. All populations were isolated in triplicates from two bilogical replicas Hence, RNA was prepared from 18 samples and was then subject to labeling and hybridization on microarray chips.

2014-06-01 | E-GEOD-49443 | biostudies-arrayexpress

Transcriptome analysis of early and late pachytene spermatocytes in WT and Zfp541-KO mice (Smart-Seq2)

2021-04-16 | GSE172171 | GEO

Round Spermatids and Pachytene Spermatocytes

Project description:Mouse Round Spermatids (Sptd) and Pachytene Spermatocytes (Spcy) Keywords: repeat sample

2005-03-15 | GSE1768 | GEO

Round Spermatids and Pachytene Spermatocytes

Project description:Mouse Round Spermatids (Sptd) and Pachytene Spermatocytes (Spcy)

2010-06-10 | E-GEOD-1768 | biostudies-arrayexpress

NPM1 chromatin binding in mouse pachytene spermatocytes [CUT&Tag]

Project description:We report the genome-wide NPM1-associated chromatin in mouse pachytene spermatocytes using ChIP-seq following immunoprecipitation with an anti-NPM1 antibody.

2026-05-28 | GSE328327 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data