Myeloid Cell States in Influenza-Associated Pulmonary Aspergillosis are Shaped by Iron Overload and Metabolic Reprogramming

ABSTRACT: We performed single-cell RNA sequencing using the 10x Genomics platform to define how prior influenza A virus (IAV) infection reshapes pulmonary monocyte and macrophage responses during secondary Aspergillus fumigatus infection in a murine model of influenza-associated pulmonary aspergillosis (IAPA). IAPA is a severe complication of influenza ICU-admitted patients, but the host mechanisms that drive susceptibility remain incompletely understood. To address this, we profiled CD11b+ monocyte/macrophage-enriched lung cells from naïve mice, IAV-only mice, A. fumigatus (CEA10)-only mice, and IAV+A. fumigatus (Coinfection) mice. This dataset provides a high-resolution view of how prior influenza infection reshapes innate immune cell states during secondary A. fumigatus infection. These data establish a resource for investigating innate immune dysregulation in IAPA and offer new insight into how influenza-driven remodeling of the lung myeloid compartment impairs monocyte and macrophage responses to secondary fungal challenge.

ORGANISM(S): Mus musculus

PROVIDER: GSE328605 | GEO | 2026/08/30

REPOSITORIES: GEO