Transcriptional landscape of mouse adipose tissue transfected with LHX8 siRNA

ABSTRACT: We have studied the role of LHX8 (Lim Homeobox 8) in adipocyte development. Mouse 3T3-L1 adipcoytes were used for our analyses. Cells were cultured in high glucose DMEM, supplemented with 10% FCS, 1% glutamine and 1% penicillin/streptomycin. Cells were grown to reach 70-80% confluence, and transfected with a siRNA silencer to decrease Lhx8 mRNA expression. Turbofectamine was used for cell transfection. Total RNA was isolated 48h post-transfection and used for subsequent NGS analysis.

ORGANISM(S): Mus musculus

PROVIDER: GSE329222 | GEO | 2026/05/21

REPOSITORIES: GEO