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The NIH Human Pluripotent Stem Cell Database (Agilent, mRNA)

ABSTRACT: To better understand the extent to which induced pluripotent stem cells (iPSCs) faithfully recapitulate the characteristics of embryonic stem cells (ESCs) under (undiff)erentiated condition, KSR condition and FBS condition and how both compare to somatic tissues under these conditions, we employed whole-genome transcriptome analysis on all twenty one hESC lines available on the pre-2008 NIH Human Pluripotent Stem Cell Registry, eight human iPSCs derived at NIH by retroviral transduction of human fibroblasts and twenty human somatic tissues. One standard culture protocol was used in conjunction with rigorous quality control. Expanded description of methods used and are available at: http://stemcelldb.nih.gov.

ORGANISM(S): Homo sapiens

PROVIDER: GSE32923 | GEO | 2012/10/31

SECONDARY ACCESSION(S): PRJNA155513

REPOSITORIES: GEO

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The NIH Human Pluripotent Stem Cell Database (Agilent, mRNA)

Project description:To better understand the extent to which induced pluripotent stem cells (iPSCs) faithfully recapitulate the characteristics of embryonic stem cells (ESCs) under (undiff)erentiated condition, KSR condition and FBS condition and how both compare to somatic tissues under these conditions, we employed whole-genome transcriptome analysis on all twenty one hESC lines available on the pre-2008 NIH Human Pluripotent Stem Cell Registry, eight human iPSCs derived at NIH by retroviral transduction of human fibroblasts and twenty human somatic tissues. One standard culture protocol was used in conjunction with rigorous quality control. Expanded description of methods used and are available at: http://stemcelldb.nih.gov. 230 samples: 44 human ESC (UNDIFF), 16 human iPSC (UNDIFF) 44 human ESC (KSR), 15 human iPSC (KSR), 44 human ESC (FBS), 15 human iPSC (FBS), 1 human Brain, 1 human Placenta, 1 human Skeletal Muscle, 1 human Heart, 1 human Liver, 1 human Lung, 1 human Kidney, 1 human Esophagus, 1 human Ovary, 1 human Testes, 1 human Spleen, 1 human Adipose, 1 human Thyroid, 1 human Thymus, 1 human Small Intestine, 1 human Prostate, 1 human Cervix, 1 human Colon, 1 human Bladder, 1 human Trachea) and 32 processing controls (UniRef).

2012-10-31 | E-GEOD-32923 | biostudies-arrayexpress

The NIH Human Pluripotent Stem Cell Database (Agilent, miRNA)

Project description:MicroRNA profiling was performed on RNA samples matched to those included in the NIH Human Pluripotent Stem Cell Database (Series GSE32923). Twenty undifferentiated human embryonic stem cell lines and 4 human tissues were analyzed. Expanded descriptions of methods used are available at: http://stemcelldb.nih.gov.

2012-10-31 | GSE34199 | GEO

The NIH Human Pluripotent Stem Cell Database (Agilent, cgh)

2012-10-31 | GSE35735 | GEO

The NIH Human Pluripotent Stem Cell Database (Agilent, miRNA)

2012-10-31 | E-GEOD-34199 | biostudies-arrayexpress

The NIH Human Pluripotent Stem Cell Database (Agilent, cgh)

Project description:Array comparative genomic hybridization was performed on DNA samples matched to the RNA samples included in the NIH Human Pluripotent Stem Cell Database (Series GSE32923). Twenty two undifferentiated human embryonic stem cell lines were analyzed. Expanded descriptions of methods used are available at: http://stemcelldb.nih.gov. 22 hybridizations: 10 human male ESC (UNDIFFerentiated) lines vs human male reference, 12 human female ESC (UNDIFFerentiated) lines vs human female reference.

2012-10-31 | E-GEOD-35735 | biostudies-arrayexpress

The NIH Human Pluripotent Stem Cell Database (Illumina, snp)

Project description:Genotyping was performed on DNA samples matched to the RNA samples included in the NIH Human Pluripotent Stem Cell Database (Series GSE32923). Seventeen undifferentiated human embryonic stem cell lines and 11 human induced pluripotent stem cells were analyzed. Expanded descriptions of methods used are available at: http://stemcelldb.nih.gov.

2012-10-31 | GSE35157 | GEO

The NIH Human Pluripotent Stem Cell Database (Illumina, methylation)

Project description:Methylation profiling was performed on DNA samples matched to the RNA samples included in the NIH Human Pluripotent Stem Cell Database (Series GSE32923). Nineteen undifferentiated human embryonic stem cell lines and 5 human induced pluripotent stem cells were analyzed. Expanded descriptions of methods used are available at: http://stemcelldb.nih.gov.

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2012-10-31 | E-GEOD-35157 | biostudies-arrayexpress

The NIH Human Pluripotent Stem Cell Database (Affymetrix, mRNA)

Project description:To broaden the appeal of the NIH Stem Cell Database, we analyzed a subset of undifferentiated human embryonic stem cell lines (5 lines in duplicate) on the Affymetrix platform. One standard culture protocol was used in conjunction with rigorous quality control. Expanded description of methods used and are available at: http://stemcelldb.nih.gov.

2012-10-31 | GSE33789 | GEO

The NIH Human Pluripotent Stem Cell Database (Illumina, methylation)

2012-10-31 | E-GEOD-34869 | biostudies-arrayexpress

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