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Transcriptional regulation of brain gene expression in response to a territorial intrusion

ABSTRACT: We investigated the transcriptomic response to territorial intrusion in four regions of the brain (telencephalon, diencephalon, cerebellum and brain stem) of a teleost fish, the threespined stickleback (Gasterosteus aculeatus). We tested the hypothesis that gene regulatory networks modulate the transcriptional response to territorial intrusion in the different brain regions and thereby aggressive behavior. Indeed, transcription regulatory network analysis identified cis-regulatory transcription factor motifs enriched in upregulated genes in one brain region and downregulated in the others. We further validated our findings by showing that many of the differentially expressed genes in the diencephalon were positively correlated with levels of aggression in individual males.

ORGANISM(S): Gasterosteus aculeatus

PROVIDER: GSE32961 | GEO | 2012/11/21

SECONDARY ACCESSION(S): PRJNA146411

REPOSITORIES: GEO

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Transcriptional regulation of brain gene expression in response to a territorial intrusion

Project description:We investigated the transcriptomic response to territorial intrusion in four regions of the brain (telencephalon, diencephalon, cerebellum and brain stem) of a teleost fish, the threespined stickleback (Gasterosteus aculeatus). We tested the hypothesis that gene regulatory networks modulate the transcriptional response to territorial intrusion in the different brain regions and thereby aggressive behavior. Indeed, transcription regulatory network analysis identified cis-regulatory transcription factor motifs enriched in upregulated genes in one brain region and downregulated in the others. We further validated our findings by showing that many of the differentially expressed genes in the diencephalon were positively correlated with levels of aggression in individual males. 2 conditions: control fish and fish that experienced intrusion into their territory, Biological replicates 5.Four brain regions. Sample 4BS was excluded from the analysis during data preprocessing because it was an outlier.

2012-11-21 | E-GEOD-32961 | biostudies-arrayexpress

Evidence for conflict at the molecular level, and a possible solution to it - gene regulation

Project description:We investigated the transcriptomic response to courtship opportunity in four regions of the brain (telencephalon, diencephalon, cerebellum and brain stem) of a teleost fish, the threespined stickleback (Gasterosteus aculeatus). We tested the hypothesis that gene regulatory networks modulate the transcriptional response tocourtship opportunity in the different brain regions and thereby mating. Indeed, transcription regulatory network analysis identified cis-regulatory transcription factor motifs enriched in upregulated genes in one brain region and downregulated in the others. We further validated our findings by qPCR.

2016-10-12 | GSE74051 | GEO

Natural variation in brain gene expression profiles of aggressive and nonaggressive individual sticklebacks

Project description:We examine whether there are differences in gene expression between aggressive versus nonaggressive behavioral types of individuals within a natural population of male threespined sticklebacks (Gasterosteus aculeatus). We compared gene expression profiles of aggressive male sticklebacks to nonaggressive males in four regions of the brain (brainstem, cerebellum, diencephalon and telencephalon). We identified new candidate genes related to aggressiveness, including SLC38a, a solute carrier which has been linked to GABAergic neuron reuptake of glutamine.

2017-02-28 | GSE78861 | GEO

Microarray transcriptional profiling of three-spined stickleback (Gasterosteus aculeatus) exposed to 10 different model toxicants and 26 mixtures of these toxicants

Project description:Adult female three-spined stickleback fish (Gasterosteus aculeatus) were exposed to 10 individual chemicals, 26 mixtures of these chemicals, or control conditions in a flow-through system for 4 days. Transcriptomics was performed on liver samples by microarray. The main aims were to determine molecular signatures induced by these chemicals in the three-spined stickleback, discover whether these persisted in chemical mixtures and identify non-additive molecular responses in chemical mixtures exposures.

2018-03-01 | E-MTAB-5581 | biostudies-arrayexpress

Temporal Dynamics of Neurogenomic Plasticity in Response to Social Interactions in Male Threespined Sticklebacks [RNA-Seq]

Project description:Animals exhibit dramatic immediate behavioral plasticity in response to social interactions, and brief social interactions can shape the future social landscape. However, the molecular mechanisms contributing to behavioral plasticity are unclear. Here, we show that the genome dynamically responds to social interactions with multiple waves of transcription associated with distinct molecular functions in the brain of male threespined sticklebacks, a species famous for its behavioral repertoire and evolution. Some biological functions (e.g., hormone activity) peaked soon after a brief territorial challenge and then declined, while others (e.g., immune response) peaked hours afterwards. We identify transcription factors that are predicted to coordinate waves of transcription associated with different components of behavioral plasticity. Next, using H3K27Ac as a marker of chromatin accessibility, we show that a brief territorial intrusion was sufficient to cause rapid and dramatic changes in the epigenome. Finally, we integrate the time course brain gene expression data with a transcriptional regulatory network, and link gene expression to changes in chromatin accessibility. This study reveals rapid and dramatic epigenomic plasticity in response to a brief, highly consequential social interaction.

2017-06-02 | GSE96673 | GEO

Temporal Dynamics of Neurogenomic Plasticity in Response to Social Interactions in Male Threespined Sticklebacks [ChIP-Seq]

2017-06-02 | GSE97370 | GEO

Proteomic analysis of the secretome of Schistocephalus solidus LC-MS/MS

Project description:Schistocephalus solidus is a cestode parasite that is thought to manipulate the behaviour of its threespine stickleback host Gasterosteus aculeatus. It has been hypothesized that the worm could liberate in its external environment “manipulation factors” that would ultimately interfere with the host’s physiology and behaviour. The objective of this project is to describe the whole proteomic content of the proteome and of the secretome of a putative manipulative parasite, Schistocephalus solidus, with the aim to identify proteins that could be involved in the behavioural perturbations of the threespine stickleback.

2021-09-09 | PXD016844 | Pride

The protein profiles of washes from the body cavity of Gasterosteus aculeatus L., infected with the Schistocephalus solidus, and from the surface of the helminth

Project description:The aim of this study was to describe the excretory–secretory proteins from the helminth Schistocephalus solidus and its intermediate host, the three-spined stickleback Gasterosteus aculeatus L., which are likely to be involved in interactions between them. Combined samples of washes from the G. aculeatus sticklebacks cavity infected with the S. solidus, and washes from the parasite surface were used as experimental samples (samples 5 to 8), while washes from the uninfected fish body cavity were used as control (samples 1 to 4). The obtained samples were analyzed using mass-spectrometry nLC–MS/MS.

2024-02-02 | PXD049088 | Pride

Project description:Gasterosteus aculeatus (three-spined stickleback)

| PRJNA779581 | ENA

Genome-wide DNA methylation profiling reveals epigenetic adaptation of stickleback to sea and freshwater conditions

Project description:Three-spined stickleback (Gasterosteus aculeatus) represents a convenient model to study microevolution - adaptation to freshwater environment. While genetic adaptations to freshwater are well-studied, epigenetic adaptations attracted little attention. In this work, we investigated the role of DNA methylation in the adaptation of marine stickleback population to freshwater conditions. DNA methylation profiling was performed in marine and freshwater populations of sticklebacks, as well as in marine sticklebacks placed into freshwater environment and freshwater sticklebacks placed into seawater. For the first time, we demonstrated that genes encoding ion channels kcnd3, cacna1fb, gja3 are differentially methylated between marine and freshwater populations. We also showed that after placing marine stickleback into fresh water, its DNA methylation profile partially converges to the one of a freshwater stickleback. This suggests that immediate epigenetic response to freshwater conditions can be maintained in freshwater population. Interestingly, we observed enhanced epigenetic plasticity in freshwater sticklebacks that may serve as a compensatory regulatory mechanism for the lack of genetic variation in the freshwater population. Some of the regions that were reported previously to be under selection in freshwater populations also show differential methylation. Thus, epigenetic changes might represent a parallel mechanism of adaptation along with genetic selection in freshwater environment.

2017-05-30 | GSE82310 | GEO

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