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Gene expression profiling during Ara-C induced NB4 cell apoptosis

ABSTRACT: As the most important antimetabolite used to induce remission in children and adults with acute leukemia, cytosine arabinoside (Ara-C) is widely used in consolidation therapy of APL to reduce the relapse rate. In order to better understand the molecular mechanism underlying Ara-C induced cell early apoptosis, we examined the mRNA level changes of NB4 cells after Ara-C treatment over the 12 h time course using a human whole genome oligo-array.

ORGANISM(S): Homo sapiens

PROVIDER: GSE32992 | GEO | 2011/10/15

SECONDARY ACCESSION(S): PRJNA146455

REPOSITORIES: GEO

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Gene expression profiling during Ara-C induced NB4 cell apoptosis

Project description:As the most important antimetabolite used to induce remission in children and adults with acute leukemia, cytosine arabinoside (Ara-C) is widely used in consolidation therapy of APL to reduce the relapse rate. In order to better understand the molecular mechanism underlying Ara-C induced cell early apoptosis, we examined the mRNA level changes of NB4 cells after Ara-C treatment over the 12 h time course using a human whole genome oligo-array. For each test and control sample at the 2, 4, 6, and 12 h time point, hybridization experiments were performed using a dye-swap strategy.

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Expression data from NB4 control shRNA cells and NB4 TRIB3 shRNA cells

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NB4 cell line untreated and induced to differentiate by a 48h exposure to 1µM ATRA

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