Induced transcription in a gene desert region advanced replication timing
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ABSTRACT: This study investigates whether transcription can directly reorganize DNA replication timing. To isolate the effect of transcription from native chromatin context, we established an inducible transcription system within a large late-replicating gene desert on mouse chromosome 2. Using Repli-seq, we found that induced transcription is sufficient to advance replication timing across an approximately 800-kb genomic domain. The induced early-replicating domain displayed an asymmetric replication timing profile relative to the knock-in site, and this asymmetry was independent of transcriptional direction. Genome-wide analysis further showed that pronounced asymmetry is uncommon among native early-replicating domains. These data provide direct evidence that transcription can reshape large-scale replication timing.
ORGANISM(S): Mus musculus
PROVIDER: GSE329934 | GEO | 2026/07/25
REPOSITORIES: GEO
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